Transcriptome-wide identification of WRKY transcription factor and their expression profiles under salt stress in sweetpotato (Ipomoea batatas L.)

文献类型： 外文期刊

第一作者： Qin, Zhen

作者： Qin, Zhen;Hou, Fuyun;Li, Aixian;Dong, Shuxu;Wang, Qingmei;Zhang, Liming;Qin, Zhen;Hou, Fuyun;Li, Aixian;Dong, Shuxu;Wang, Qingmei;Zhang, Liming;Zhang, Liming

作者机构：

关键词： Sweetpotato; WRKY transcription factor; Transcriptome; Salt stress

期刊名称：PLANT BIOTECHNOLOGY REPORTS （ 影响因子：2.01； 五年影响因子：1.907 ）

ISSN： 1863-5466

年卷期： 2020 年 14 卷 5 期

页码：

收录情况： SCI

摘要： WRKY transcription factors play a key role in regulating plant growth, development, and stress responses. Although many WRKY transcription factors have been discovered in plants, the WRKY transcription factors in sweetpotato have not been systematically studied. Here, we identified 79 IbWRKY transcription factors based on the transcriptome data of sweetpotato. The amino acid length of the protein ranged from 162 to 711 amino acids. Frequency analysis of amino acids in IbWRKY transcription factors found highest levels of serine. Subcellular localization analysis found 77 IbWRKY proteins localized in the nucleus. The IbWRKY proteins were clustered into three groups based on their structural and phylogenetic characteristics. Seven WRKY proteins contained the WRKY domain variations WRKYG(K/T)K as revealed by multiple sequence alignment. Structural analysis showed that IbWRKY proteins of the same subfamily had similar motifs. Transcriptome data analysis found that a total of 35IbWRKYgenes showed significant changes of the expression level after the 150 mM NaCl treatment. We selected 16IbWRKYgenes for real-time quantitative PCR verification and found that the data were consistent with the transcriptome data. In summary, this study provides a systematical analysis of the IbWRKY transcription factors, which will lay a solid foundation for further characterization of the regulatory mechanism of theIbWRKYgenes under salt stress in sweetpotato.

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