An Enhanced Variant Designed From DLP4 Cationic Peptide AgainstStaphylococcus aureusCVCC 546

文献类型： 外文期刊

第一作者： Li, Bing

作者： Li, Bing;Yang, Na;Wang, Xiumin;Hao, Ya;Mao, Ruoyu;Li, Zhanzhan;Wang, Zhenlong;Teng, Da;Wang, Jianhua;Li, Bing;Yang, Na;Wang, Xiumin;Hao, Ya;Mao, Ruoyu;Li, Zhanzhan;Wang, Zhenlong;Teng, Da;Wang, Jianhua

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关键词： insect defensin; peptide design; bioavailability; Staphylococcus aureus; antimicrobial mechanism; pharmacodynamics

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： Insect defensins are promising candidates for the development of potent antimicrobials against antibiotic-resistantStaphylococcus aureus(S. aureus). An insect defensin, DLP4, isolated from the hemolymph ofHermetia illucenslarvae, showed low antimicrobial activity against Gram-positive (G(+)) pathogens and high cytotoxicity, which limited its effective therapeutic application. To obtain more potent and low cytotoxicity molecules, a series of peptides was designed based on the DLP4 template by changing the conservative site, secondary structure, charge, or hydrophobicity. Among them, a variant designated as ID13 exhibited strong antibacterial activity at low MIC values of 4-8 mu g/mL to G(+)pathogens (S. aureus: 4 mu g/mL;Staphylococcus epidermidis: 8 mu g/mL;Streptococcus pneumoniae: 4 mu g/mL;Streptococcus suis: 4 mu g/mL), which were lower than those of DLP4 (S. aureus: 16 mu g/mL;S. epidermidis: 64 mu g/mL;S. pneumoniae: 32 mu g/mL;S. suis: 16 mu g/mL), and cytotoxicity of ID13 (71.4% viability) was less than that of DLP4 (63.8% viability). ID13 could penetrate and destroy the cell membrane ofS. aureusCVCC 546, resulting in an increase in potassium ion leakage; it bound to genomic DNA (gDNA) and led to the change of gDNA conformation. After treatment with ID13, perforated, wrinkled, and collapsedS. aureusCVCC 546 cells were observed in electron microscopy. Additionally, ID13 killed over 99.99% ofS. aureuswithin 1 h, 2 x MIC of ID13 induced a post-antibiotic effect (PAE) of 12.78 +/- 0.28 h, and 10 mg/kg ID13 caused a 1.8 log(10)(CFU/g) (CFU: colony-forming units) reduction ofS. aureusin infected mouse thigh muscles and a downregulation of TNF-alpha, IL-6, and IL-10 levels, which were superior to those of DLP4 or vancomycin. These findings indicate that ID13 may be a promising peptide antimicrobial agent for therapeutic application.

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