Extracellular Nanovesicle Enhanced Gene Transfection Using Polyethyleneimine in HEK293T Cells and Zebrafish Embryos

文献类型： 外文期刊

第一作者： Zhang, Zhenzhen

作者： Zhang, Zhenzhen;Wen, Kai;Wang, Zhenglong;Zhou, Xiang;Zhang, Zhenzhen;Zhang, Chao;Laroche, Fabrice;Zhou, Qiang;Liu, Zunfeng;Zhou, Xiang;Abrahams, Jan Pieter

作者机构：

关键词： gene transfection; extracellular nanovesicles; zebra fish; polyethyleneimine; efficiency

期刊名称：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY （ 影响因子：5.89； 五年影响因子：5.973 ）

ISSN： 2296-4185

年卷期： 2020 年 8 卷

页码：

收录情况： SCI

摘要： It is a hot topic to improve efficiency and decrease toxicity of gene transfection reagents. The extracellular nanovesicles (EVs) that are released by cells play an important role in intercellular communication and are naturally designed for genetic exchange between cells. Here, we show that the EVs have a large beneficial effect in polyethyleneimine (PEI)-mediated transfection of a GFP-encoding plasmid into HEK293T cells. An improvement of transfection efficiency of similar to 500% and a decrease in toxicity were observed in a specific concentration range of PEI. The EVs also greatly improved the transfection of the same plasmid into zebrafish embryos. To verify the generality of this gene transfection approach, we also tested the cell viability and gene transfection efficiency using two other plasmids (EpTEN and ELuc) and in another cell line (A549). The measured increase in transfection efficiency makes EV a promising candidate for enhancement of the quality of current PEI-based transfection technique.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序