Effects of osmotic stress on the expression profiling of aquaporin genes in the roughskin sculpin (Trachidermus fasciatus)

文献类型： 外文期刊

第一作者： Ma, Qian

作者： Ma, Qian;Ma, Qian;Liu, Xinfu;Li, Ang;Liu, Shufang;Zhuang, Zhimeng

作者机构：

关键词： Trachidermus fasciatus; aquaporin; salinity changes; stress response; gene expression

期刊名称：ACTA OCEANOLOGICA SINICA （ 影响因子：1.431； 五年影响因子：1.445 ）

ISSN： 0253-505X

年卷期： 2020 年 39 卷 6 期

页码：

收录情况： SCI

摘要： Aquaporins (AQPs) are a family of integral membrane proteins that have been shown to be important for osmoregulation in many vertebrates. To identify potential stress resistance-relatedaqpgenes in salinity adaptation of the roughskin sculpinTrachidermus fasciatus, we investigated the time-course expression dynamics of seven aquaporin genes (aqpl, 4, 7, 8, 10, 11and12) in three osmoregulatory tissues (kidney, gill and intestine) and one metabolic tissue (liver). The fish were subjected to two different acute osmotic treatments (seawater-to-freshwater transfer respectively achieved in 1 h and 24 h, namely, E-acute and acute group). The expression profiling of the sevenaqpgenes were performed using quantitative real-time PCR (qRT-PCR). At the time of all sampling time points (0 h, 12 h, 24 h and 48 h), no expression ofaqp4was found in the gill, liver and intestine; no expression ofaqp7was found in the gill and liver. Significant differences ofaqpexpression were determined in the four target tissues, and the mRNA levels were largely variable among gene members and tissues. Similar patterns of the time-course expression were detected in most of theaqpgenes inT. fasciatusbetween the two acute groups, except that only one gene (aqpl2) in the kidney and three genes (aqp7, aqp8andaqp10) in the intestine revealed different expression patterns. These results suggest that the expression response ofaqpgenes was similar under osmotic changes with different rates.

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