A new approach to protect tobacco plants from Cd contamination using the attenuated recombinant virus CMVo2b containing thePvSR2gene

文献类型： 外文期刊

第一作者： Wu, Juan

作者： Wu, Juan;Wang, Qiang;Zhu, Xiwu;Wu, Juan;Wang, Qiang

作者机构：

关键词： CMVo2b; PvSR2 gene; Cd; Nicotiana benthamiana; virus vector

期刊名称：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT （ 影响因子：1.632； 五年影响因子：2.029 ）

ISSN： 1310-2818

年卷期： 2020 年 34 卷 1 期

页码：

收录情况： SCI

摘要： Reducing the Cd content in crop plants is an important process for ensuring the safety of food made from the crop. In this study, the CMVo2b vector was used to express thePvSR2gene to reduce the Cd content ofNicotiana benthamianaplants. The infectious clone plasmid pCB301-CMVFny209o2b-PvSR2 was successfully constructed.N. benthamianaplants at six leaf stages were inoculated with plasmid-Agrobacterium (GV3101) containing pCB301-CMVFny209o2b-PvSR2, pCB301-CMVFny109 and pCB301-CMVFny309 by agroinfiltration. After the virus had been detected in the non-inoculated leaves of the plant inoculated with plasmid-agrobacterium corresponding to each treatment, the plant of each treatments in the experiment were inoculated with sap of plant leaves containing the virus. After 8 days, the plants were treated with Cd(++)solution. The Cd content of the plant samples was analyzedviainductively coupled plasma atomic emission spectrometry. The results show that the plants expressing thePvSR2gene had greatly reduced Cd(++)content than the control and grow normally. These results indicated that using the attenuated recombinant virus CMVo2b containing thePvSR2gene significantly enhanced the resistance of the plant to Cd++. This is a new approach to protect tobacco plants from Cd contamination by using the attenuated recombinant virus CMVo2b containing thePvSR2gene.

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