Molecular cloning, characterization and tissue specificity of the expression in the TAC1 genes from Henan Huai goat (Capra hircus)

文献类型： 外文期刊

第一作者： Tian, Zhilong

作者： Tian, Zhilong;Wang, Yuqin;Shi, Huibin;Wu, Zhibo;Zhang, Xiaohui;Wang, Jianping;Li, Yuanxiao;Yang, Fang;Liu, Yumei;Wang, Yuqin;Tian, Zhilong;Chu, Mingxing

作者机构：

关键词： Clone and sequence analysis; Expression; Goat; Tachykinin

期刊名称：INDIAN JOURNAL OF ANIMAL RESEARCH （ 影响因子：0.44； 五年影响因子：0.543 ）

ISSN： 0367-6722

年卷期： 2020 年 54 卷 6 期

页码：

收录情况： SCI

摘要： To further to understand the structure and function of the TAC1 gene, we cloned the full-length cDNAs of the TAC1 genes from goat by rapid amplification of cDNA ends-PCR and the qRT-PCR was used to analyze the TAC1 mRNA expression patterns of goat various tissues. The full-length cDNA of goat TAC1 was 1176 bp, with a 339 bp open reading frame encoding 112 amino acids. The amino acid sequence analysis revealed that goat TAC1 gene encoded a water-drain protein and its relative molecular weight and isoelectric point was 13,012.86 Da and 6.29 respectively. Alignment and phylogenetic analyses revealed that their amino acid sequences were highly similar to those of other vertebrates. TAC1 expression of the goat of the brain, cerebellum, medulla oblongata, heart, liver, spleen, lung, kidney, uterus, ovaries. These results serve as a foundation for further study on the Capra hircus TAC1 gene.

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