文献类型： 外文期刊

第一作者： Hai-ying, Guan

作者： Hai-ying, Guan;Shou-ping, Lu;Tie-shan, Liu;Chun-mei, He;Chun-xiao, Liu;Qiang, Liu;Rui, Dong;Juan, Wang;Shi-jun, Qi;Li-ming, Wang;Yong-bin, Dong;Yu-ling, Li

作者机构：

关键词： miniature2-m1; nitrate transporter1.5; frame shift mutation; allelism tests; subcellular localization

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2020 年 19 卷 8 期

页码：

收录情况： SCI

摘要： Kernel development plays an important role in determining kernel size in maize. Here we present the cloning and characterization of a maize gene, nitrate transporter1.5 ( NRT1.5 ), which controls small kernel phenotype by playing an important role in kernel development. A novel recessive small kernel mutant miniature2 - m1 ( mn2-m1 ) was isolated from self -pollinated progenies of breeding materials. The mutant spontaneously showed small kernel character arresting both embryo and endosperm development at an early stage after pollination. Utilizing 21 polymorphic SSR markers, the mn2-m1 locus was limited to a 209.9 -kb interval using 9 176 recessive individuals of a BC 1 segregating population from mn2-m1 /B73. Only one annotated gene was located in this 209.9 kb region, Zm00001d019294 , which was predicted to encode nitrate transporter1.5 (NRT1.5). Allelism tests confirmed that mn2-m1 was allelic to miniature2 - m2 ( mn2-m2 ) and miniature2-710B ( mn2-710B ). The mn2-m1 and mn2-m2 alleles both had nucleotide deletions in the coding region resulting in premature termination, and the mn2-710B allele had some missence mutations. Subcellular localization showed that Miniature 2 (MN2) is localized in the plasma membrane. Quantitative real-time PCR (qRT-PCR) analysis revealed that the expression of MN2 and some genes involved in the basal endosperm transfer layer (BETL) and embryo surrounding region (ESR) development were affected in mn2-m1 seeds. These results suggested that MN2 plays an important role in maize seed development.

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