文献类型： 外文期刊

第一作者： Yan-ju, Shan

作者： Yan-ju, Shan;Gai-ge, Ji;Jian-min, Zou;Ming, Zhang;Yun-jie, Tu;Yi-fan, Liu;Xiao-jun, Ju;Jing-ting, Shu

作者机构：

关键词： PGC-1 alpha gene; chicken; myofiber type specificity; mRNA expression profile

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2020 年 19 卷 8 期

页码：

收录情况： SCI

摘要： Previous studies on mammals showed that peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1 alpha) played a prominent role in regulating muscle fiber type transition and composition. However, the role of PGC-1 alpha in chicken muscle has seldom been explored. To investigate the effect of PGC-1 alpha on chicken skeletal muscles in this study, the PGC-1 alpha gene was overexpressed or silenced in chicken primary myoblasts by using lentivirus, and then the effects of the PGC-1 alpha gene overexpression and knockdown on the mRNA expression profile of genes related to myofiber type specificity were examined during fiber formation. The results showed that overexpression of PGC-1 alpha from proliferation to differentiation was accompanied by the up -regulated expression of Pax7 , MyoD , and CnA? , which was significantly (P< 0.01) increased after one day of transfection (1I). The enhancement of MyoG , MEF2c , and MyHC SM expression lagged, which was improved significantly (P< 0.01) after four days of transfection (1I3D). Overexpression of PGC-1 alpha decreased (P 0.01) the MyHC FWM expression after four days of transfection (1I3D), and it had no significant impact (P 0.01) up -regulated the expression of MyHC FWM , and had no significant impact (P 0.05) on the expression of CnB1 , NFATc3 , and MyHC FRM . These results indicated that the role of PGC-1 alpha in regulating the fiber type specificity of chicken skeletal muscles might be similar to that in mammals, which interplayed with key genes related to myocyte differentiation and calcineurin signaling pathway.

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