CRISPR/Cas9-mediated knockout and overexpression studies reveal a role of maize phytochrome C in regulating flowering time and plant height

文献类型： 外文期刊

第一作者： Li, Quanquan

作者： Li, Quanquan;Chen, Cuixia;Wu, Guangxia;Zhao, Yongping;Wang, Baobao;Zhao, Binbin;Kong, Dexin;Wei, Hongbin;Wang, Haiyang;Wang, Haiyang

作者机构：

关键词： maize; phytochrome C; flowering time; shade-avoidance syndrome; high-density planting

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：9.803； 五年影响因子：9.555 ）

ISSN： 1467-7644

年卷期：

页码：

收录情况： SCI

摘要： Maize is a major staple crop widely used for food, feedstocks and industrial products. Shade-avoidance syndrome (SAS), which is triggered when plants sense competition of light from neighbouring vegetation, is detrimental for maize yield production under high-density planting conditions. Previous studies have shown that the red and far-red photoreceptor phytochromes are responsible for perceiving the shading signals and triggering SAS in Arabidopsis; however, their roles in maize are less clear. In this study, we examined the expression patterns ofZmPHYC1andZmPHYC2and found thatZmPHYC1, but notZmPHYC2, is highly expressed in leaves and is regulated by the circadian clock. Both ZmPHYC1 and ZmPHYC2 proteins are localized to both the nucleus and cytoplasm under light conditions and both of them can interact with themselves or with ZmPHYBs. Heterologous expression ofZmPHYCscan complement the ArabidopsisphyC-2mutant under constant red light conditions and confer an attenuated SAS in Arabidopsis in response to shading. Double knockout mutants ofZmPHYC1andZmPHYC2created using the CRISPR/Cas9 technology display a moderate early-flowering phenotype under long-day conditions, whereasZmPHYC2overexpression plants exhibit a moderately reduced plant height and ear height. Together, these results provided new insight into the function ofZmPHYCsand guidance for breeding high-density tolerant maize cultivars.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序