Protective effect of vitamin C and lycopene on the in vitro fertilization capacity of sex-sorted bull sperm by inhibiting the oxidative stress
文献类型： 外文期刊
第一作者： Zhang, Pei-Pei
作者： Zhang, Pei-Pei;Wang, Jing-Jing;Li, Chong-Yang;Hao, Hai-sheng;Wang, Hao-Yu;Du, Wei-Hua;Pang, Yun-Wei;Zhao, Shan-Jiang;Zou, Hui-Ying;Hao, Tong;Yang, Sha;Zhu, Hua-Bin;Zhao, Xue-Ming;Zhang, Pei-Pei;Wang, Jing-Jing;Li, Chong-Yang;Hao, Hai-sheng;Wang, Hao-Yu;Du, Wei-Hua;Pang, Yun-Wei;Zhao, Shan-Jiang;Zou, Hui-Ying;Hao, Tong;Yang, Sha;Zhu, Hua-Bin;Zhao, Xue-Ming;Ruan, Wei-Min;Huang, Jin-Ming;Jiao, Peng-cheng
作者机构：
关键词： in vitro fertilization; oxidative stress; sexual selection; sperm
期刊名称：REPRODUCTION IN DOMESTIC ANIMALS （ 影响因子：2.005； 五年影响因子：2.154 ）
ISSN： 0936-6768
年卷期： 2020 年 55 卷 9 期
页码：
收录情况： SCI
摘要： The fertilization capacity of sex-sorted sperms is seriously decreased, which inhibits its wide application. However, little information is still available about the effect of vitamin C (VC) and lycopene (Lyc) on the fertilization capacity of sex-sorted bull sperm. In this study, the washing medium and fertilization medium of sex-sorted sperm from three bull individuals were supplemented with different concentrations of VC (0, 1 x 10(-3), 1 x 10(-4), 1 x 10(-5), 1 x 10(-6) M) or Lyc (0, 1 x 10(-4), 1 x 10(-5), 1 x 10(-6), 1 x 10(-7)). After washing twice and incubation for 1.5 hr, the malondialdehyde (MDA) level, phosphatidylserine (PS) translocation, membrane potential (Delta psi m) and IVF (in vitro fertilization) ability of sex-sorted sperm were investigated. For the sex-sorted sperm of bulls A, B and C, 1 x 10(-3) M VC or 1 x 10(-4) M Lyc treatment significantly decreased their MDA levels and PS translocation and increased their Delta psi m levels and cleavage rates after IVF. When blastocysts were concerned, 1 x 10(-4) M Lyc significantly improved the blastocyst rates and their IFN-tau expression of bulls A and C. In conclusion, supplementation of 1 x 10(-3) M VC or 1 x 10(-4) M Lyc in washing and fertilization medium contributed greatly to improving the fertilization capacity of sex-sorted bull sperm during IVF procedure.
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