Development of novel EST microsatellite markers for genetic diversity analysis and correlation analysis of velvet antler growth characteristics in Sika deer

文献类型： 外文期刊

第一作者： Jia, Boyin

作者： Jia, Boyin;Zhang, Jiali;Liu, Yuan;Li, Qining;Wang, Guiwu;Zheng, Junjun;Yang, Wanyun;Chang, Shuzhuo;Yang, Fuhe;Wang, Guiwu;Zheng, Junjun;Yang, Wanyun;Chang, Shuzhuo;Yang, Fuhe;Ge, Chenxia;Chen, Guang;Liu, Dongdong;Liu, Dongdong

作者机构：

关键词： EST microsatellite markers; Genetic diversity; Sika deer; Transcriptome; Velvet antler

期刊名称：HEREDITAS （ 影响因子：3.271； 五年影响因子：2.807 ）

ISSN： 1601-5223

年卷期： 2020 年 157 卷 1 期

页码：

收录情况： SCI

摘要： Background Sika deer is one of the most popular and valued animals in China. However, few studies have been conducted on the microsatellite of Sika deer, which has hampered the progress of genetic selection breeding. To develop and characterize a set of microsatellites for Sika deer which provide helpful information for protection of Sika deer natural resources and effectively increase the yield and quantity of velvet antler. Results We conducted a transcriptome survey of Sika deer using next-generation sequencing technology. One hundred eighty-two thousand two hundred ninety-five microsatellite markers were identified in the transcriptome, 170 of 200 loci were successfully amplified across panels of 140 individuals from Shuangyang Sika deer population. And 29 loci were found to be obvious polymorphic. Number of alleles is from 3 to 14. The expected heterozygosity ranged from 0.3087 to 0.7644. The observed heterozygosity ranged from 0 to 0.7698. The polymorphism information content values of those microsatellites varied ranged from 0.2602 to 0.7507. The marker-trait association was tested for 6 important and kernel characteristics of two-branched velvet antler in Shuangyang Sika deer through one-way analysis of variance. The results showed that marker-trait associations were identified with 8 different markers, especially M009 and M027. Conclusions This study not only provided a large scale of microsatellites which were valuable for future genetic mapping and trait association in Sika deer, but also offers available information for molecular breeding in Sika deer.

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