Understanding the heat resistance of cucumber through leaf transcriptomics

文献类型： 外文期刊

第一作者： Wang, Min

作者： Wang, Min;He, Xiaoming;Liang, Zhaojun;Peng, Qingwu;Liu, Wenrui;Jiang, Biao;Xie, Dasen;Chen, Lin;Yan, Jinqiang;Lin, Yu'e;Wang, Min;He, Xiaoming;Liu, Wenrui;Jiang, Biao;Xie, Dasen;Chen, Lin;Yan, Jinqiang;Peng, Qin;Lin, Yu'e

作者机构：

关键词： DEGs; differentially expressed genes; heat stress; RNA-seq

期刊名称：FUNCTIONAL PLANT BIOLOGY （ 影响因子：3.101； 五年影响因子：3.248 ）

ISSN： 1445-4408

年卷期： 2020 年 47 卷 8 期

页码：

收录情况： SCI

摘要： Heat stress is a major environmental factor limiting plant productivity and quality in agriculture. Cucumber, one of the most important vegetables among cucurbitaceae, prefers to grow in a warm environment. Until now the molecular knowledge of heat stress in cucumber remained unclear. In this study, we performed transcriptome analysis using two diverse genetic cucumber cultivars, L-9 and A-16 grown under normal and heat stress. L-9 displayed heat-tolerance phenotype with higher superoxide dismutase enzyme (SOD) enzyme activity and lower malondialdehyde (MDA) content than A-16 under heat stress. RNA-sequencing revealed that a total of 963 and 2778 genes are differentially expressed between L-9 and A-16 under normal and heat stress respectively. In addition, we found that differentially expressed genes (DEGs) associated with plant hormones signally pathway, transcription factors, and secondary metabolites showed significantly change in expression level after heat stress, which were confirmed by quantitative real-time PCR assay. Our results not only explored several crucial genes involved in cucumber heat resistance, but also provide a new insight into studying heat stress.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序