Proteomic profiling uncovered the cytosolic superoxide dismutase BsSOD1 associated with plant defence in the herbal orchid Bletilla striata

文献类型： 外文期刊

第一作者： Lu, Bao-Wei

作者： Lu, Bao-Wei;An, Feng-Xia;Yang, Yong-Jian;Liu, Peng-Ming;Yang, Bao-Liang;Cao, Liang-Jing;Wang, Xuan;Zhang, Yu-Lei;Liu, Jun;Zhang, Yu-Lei;Ding, Yan-Feng

作者机构：

关键词： disease resistance; leaf blight; protoplast transformation

期刊名称：FUNCTIONAL PLANT BIOLOGY （ 影响因子：3.101； 五年影响因子：3.248 ）

ISSN： 1445-4408

年卷期： 2020 年 47 卷 10 期

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收录情况： SCI

摘要： The herbal orchid Bletilla striata (Thunb.) Rchb.f. has a long cultivation history and has been widely used in medicines and cosmetics. The fungal infection leaf blight (LB) seriously threatens B. striata cultivation. Here, we systemically collected wild B. striata accessions and isolated the accessions with strong resistance against LB. We carried out proteomic profiling analysis of LB-resistant and LB-susceptible accessions, and identified a large number of differentially expressed proteins with significant gene ontology enrichment for 'oxidoreductase activity.' Of the proteins identified in the reactive oxygen species signalling pathway, the protein abundance of the Cu-Zn superoxide dismutase BsSODI and its gene expression level were higher in LB-resistant accessions than in LB-susceptible lines. Transient expression of the dismutase fused with yellow fluorescent protein determined that its subcellular localisation is in the cytoplasm. Our study provides new insights into the molecular markers associated with fungal infection in B. striata.

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