ERF4affects fruit firmness through TPL4 by reducing ethylene production

文献类型： 外文期刊

第一作者： Hu, Yanan

作者： Hu, Yanan;Han, Zhenyun;Sun, Yaqiang;Wang, Shuai;Wang, Ting;Wang, Yi;Zhang, Xinzhong;Xu, Xuefeng;Han, Zhenhai;Wu, Ting;Han, Zhenyun;Xu, Kenong

作者机构：

关键词： fruit firmness; EAR motif; ERF4; ethylene; Malus

期刊名称：PLANT JOURNAL （ 影响因子：6.417； 五年影响因子：7.627 ）

ISSN： 0960-7412

年卷期： 2020 年 103 卷 3 期

页码：

收录情况： SCI

摘要： The firmness of fleshy fruit crops has a significant effect on their quality, consumer preference, shelf life and transportability. In a combined quantitative trait locus and genome-wide association studies study of apple fruit texture, we identified a mutation (C-G) in the ethylene response factor-associated amphiphilic repression (EAR) motif in the coding region of the apple ETHYLENE RESPONSE FACTOR4 (ERF4) gene. Chromatin immunoprecipitation sequencing showed that ERF4 binds to the promoter ofERF3, which is involved in regulation of ethylene biosynthesis. The EAR mutation in ERF4 results in reduced repression ofERF3expression, which is turn promotes ethylene production and loss of fruit firmness. ERF4 acts as a transcriptional repressor whose activity is modulated by a TOPLESS co-repressor 4 (TPL4)-binding EAR repression motif. Biolayer interferometry analysis showed that the mutation in the EAR motif causes a reduction in the interaction with TPL4. Suppression ofERF4orTPL4promoted fruit ripening and ethylene production. Taken together, our results provide insights into howERF4allelic variation underlies an important fruit quality trait.

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