Plant regeneration from young leaves in loquat (Eriobotrya japonicaL.) using young grafted seedlings as mother plants

文献类型： 外文期刊

第一作者： Deng, Renju

作者： Deng, Renju;Fan, Jianxin;Wang, Yongqing;Cui, Wenning

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关键词： Plant regeneration; Young leaves; Grafted seedlings; Loquat (Eriobotrya japonicaL; )

期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT （ 影响因子：2.252； 五年影响因子：2.139 ）

ISSN： 1054-5476

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收录情况： SCI

摘要： A plant regeneration technique was successfully developed for the first time using young leaves harvested from two-y-old grafted seedlings in loquat (Eriobotrya japonicaL.) cv. Dawuxing. Callus cultures were induced from the cut edge of the leaf explants after 2 wk of culture on Murashige and Skoog (MS) medium supplemented with 0.5 mg L(-1)6-benzyladenine (BA) and 0.5 mg L(-1)2,4-dichlorophenoxyacetic acid (2,4-D), in which callus cultures were induced at a frequency of 89.2%. White-greenish adventitious buds occurred after 3 wk of culture on MS medium supplemented with 0.8 mg L(-1)thidiazuron (TDZ), 0.3 mg L-1 alpha-naphthaleneacetic acid (NAA), and 0.2 mg L-1AgNO3, the adventitious buds developed to shoots after 5 wk of culture, with 30.6% of shoot induction rate. The shoots (about 5 cm in length with expanded leaves) rooted at a rate of 68.9% on the rooting medium consisting of 1/2 MS medium containing 1.0 mg L(-1)NAA and 1.0 mg L(-1)indolebutyric acid (IBA). The rooted plantlets, after hardening, survived satisfactorily in the transplantation matrix of peat and humus under optimum temperature, light, and water management. The morphology and inter simple sequence repeat (ISSR) marker banding patterns of the regenerated plants were all identical and similar to that of the mother plant. The plant regeneration technique developed in this study could be useful forAgrobacterium-mediated genetic transformation in loquat.

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