Dendrigraft poly-L-lysines delivery of DNA vaccine effectively enhances the immunogenic responses against H9N2 avian influenza virus infection in chickens

文献类型： 外文期刊

第一作者： Zhao, Kai

作者： Zhao, Kai;Rong, Guangyu;Lan, Hailing;Yu, Lu;Yu, Shuang;Zhao, Kai;Rong, Guangyu;Lan, Hailing;Yu, Lu;Yu, Shuang;Rong, Guangyu;Teng, Qiaoyang;Li, Xuesong;Lan, Hailing;Li, Zejun;Rong, Guangyu;Teng, Qiaoyang;Li, Xuesong;Lan, Hailing;Li, Zejun;Jin, Zheng;Chen, Guangping

作者机构：

关键词： H9N2 avian influenza; Dendrigraft poly-L-lysine; Nanoparticles; Delivery of DNA vaccine; Immune responses

期刊名称：NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE （ 影响因子：6.458； 五年影响因子：7.072 ）

ISSN： 1549-9634

年卷期： 2020 年 27 卷

页码：

收录情况： SCI

摘要： Biodegradable nanomaterials can protect antigens from degradation, promote cellular absorption, and enhance immune responses. We constructed a eukaryotic plasmid [pCAGGS-opti441-hemagglutinin (HA)] by inserting the optimized HA gene fragment of H9N2 AIV into the pCAGGS vector. The pCAGGS-opti441-HA/DGL was developed through packaging the pCAGGS-opti441-HA with dendrigraft poly-llysines (DCiLs). DGL not only protected the pCAGGS-opti441 -HA from degradation, but also exhibited high transfection efficiency. Strong cellular immune responses were induced in chickens immunized with the pCAGGS-opti441-HA/DGL. The levels of IFN-gamma and IL-2, and lymphocyte transformation rate of the vaccinated chickens increased at the third week post the immunization. For the vaccinated chickens, T lymphocytes were activated and proliferated, the numbers of CD3+CD4+ and CD4+/CD8+ increased, and the chickens were protected completely against H9N2 MV challenge. This study provides a method for the development of novel AIV vaccines, and a theoretical basis for the development of safe and efficient gene delivery carriers. (C) 2020 Elsevier Inc. All rights reserved.

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