Transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture

文献类型： 外文期刊

第一作者： Dong, Fu-shuang

作者： Dong, Fu-shuang;Lv, Meng-yu;Liang, Xin-xia;Liu, Yong-wei;Yang, Fan;Zhao, He;Chai, Jian-Fang;Zhou, Shuo;Wang, Jin-ping;Shi, Xue-ping

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关键词： RNA sequencing; Wheat; Immature embryo culture; Phenylpropanoid biosynthesis; Plant hormone signaling

期刊名称：BMC GENETICS （ 影响因子：2.797； 五年影响因子：3.263 ）

ISSN： 1471-2156

年卷期： 2020 年 21 卷 1 期

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收录情况： SCI

摘要： Background Activated charcoal (AC) is highly adsorbent and is often used to promote seedling growth in plant tissue culture; however, the underlying molecular mechanism remains unclear. In this study, root and leaf tissues of 10-day-old seedlings grown via immature embryo culture in the presence or absence of AC in the culture medium were subjected to global transcriptome analysis by RNA sequencing to provide insights into the effects of AC on seedling growth. Results In total, we identified 18,555 differentially expressed genes (DEGs). Of these, 11,182 were detected in the roots and 7373 in the leaves. In seedlings grown in the presence of AC, 9460 DEGs were upregulated and 7483 DEGs were downregulated in the presence of AC as compared to the control. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed 254 DEG-enriched pathways, 226 of which were common between roots and leaves. Further analysis of the major metabolic pathways revealed that AC stimulated the expression of nine genes in the phenylpropanoid biosynthesis pathway, includingPLA, CYP73A, COMT, CYP84A,and4CL, the protein products of which promote cell differentiation and seedling growth. Further, AC upregulated genes involved in plant hormone signaling related to stress resistance and disease resistance, includingEIN3, BZR1, JAR1, JAZ,andPR1, and downregulated genes related to plant growth inhibition, includingBKI1, ARR-B, DELLA,andABF. Conclusions Growth medium containing AC promotes seedling growth by increasing the expression of certain genes in the phenylpropanoid biosynthesis pathway, which are related to cell differentiation and seedling growth, as well as genes involved in plant hormone signaling, which is related to resistance.

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