Decreased Protein Abundance of Lycopene beta-Cyclase Contributes to Red Flesh in Domesticated Watermelon(1)

文献类型： 外文期刊

第一作者： Zhang, Jie

作者： Zhang, Jie;Sun, Honghe;Guo, Shaogui;Ren, Yi;Li, Maoying;Wang, Jinfang;Zhang, Haiying;Gong, Guoyi;Xu, Yong

作者机构：

期刊名称：PLANT PHYSIOLOGY （ 影响因子：8.34； 五年影响因子：8.972 ）

ISSN： 0032-0889

年卷期： 2020 年 183 卷 3 期

页码：

收录情况： SCI

摘要： The gene ClLCYB contributes to the red flesh color of domesticated watermelon through decreased protein level to convert lycopene to beta-carotene in the carotenogenesis pathway. Red-fleshed watermelons (Citrullus lanatus) that accumulate lycopene in their flesh cells have been selected and domesticated from their pale-fleshed ancestors. However, the molecular basis of this trait remains poorly understood. Using map-based cloning and transgenic analysis, we identified a lycopene beta-cyclase (ClLCYB) gene that controls the flesh color of watermelon. Down-regulation ofClLCYBcaused the flesh color to change from pale yellow to red, andClLCYBoverexpression in the red-fleshed line caused the flesh color to change to orange. Analysis ofClLCYBsingle-nucleotide polymorphisms using 211 watermelon accessions with different flesh colors revealed that two missense mutations between three haplotypes (ClLCYBred,ClLCYBwhite, andClLCYB(yellow)) were selected and largely fixed in domesticated watermelon. Proteins derived from these threeClLCYBhaplotypes were localized in plastids to catalyze the conversion of lycopene to beta-carotene and showed similar catalytic abilities. We revealed that ClLCYB protein abundance, instead ofClLCYBtranscript level, was negatively correlated with lycopene accumulation. Different amounts of ClLCYB protein degradation among theClLCYBhaplotypes were found in ClLCYB transgenic Arabidopsis (Arabidopsis thaliana) lines. After treatment with the proteasome inhibitor MG132, the concentration of ClLCYB(red)increased noticeably compared with other ClLCYB proteins. These results indicate that natural missense mutations withinClLCYBinfluence ClLCYB protein abundance and have contributed to the development of red flesh color in domesticated watermelon.

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