Microbial Community and Short-Chain Fatty Acid Mapping in the Intestinal Tract of Quail

文献类型： 外文期刊

第一作者： Du, Xizhong

作者： Du, Xizhong;Xiang, Yun;Lou, Fangfang;Tu, Pingguang;Zhang, Xiaojun;Hu, Xujin;Lyu, Wentao;Xiao, Yingping

作者机构：

关键词： quail; intestine; microbiota; short-chain fatty acids

期刊名称：ANIMALS （ 影响因子：2.752； 五年影响因子：2.942 ）

ISSN： 2076-2615

年卷期： 2020 年 10 卷 6 期

页码：

收录情况： SCI

摘要： Simple Summary Quail is an economically important type of poultry, valued for its high meat quality and abundant egg nutrition. It is also an important laboratory research animal, widely used in developmental biology and toxicology tests. Since the gut microbiota plays a vital role in the host's growth and health, we investigated the microbiota inhabiting the duodenum, jejunum, ileum, cecum, and colorectum of quail in the present study, using 16S rRNA gene sequencing and qPCR. The concentrations of short-chain fatty acids (SCFAs) were evaluated using gas chromatography. We found that the microbiota in the cecum was different from other intestinal sections and the enriched inhabitants of SCFA-producing bacterial genera made cecum the core locations of SCFA production in quail. The results of this study will provide fundamental data for further quail microbiology and functional studies. Quail is raised throughout China for egg and meat production. To deeply understand the gastrointestinal microbial composition and metabolites of quail, the present study characterized the microbiota inhabiting five intestinal locations of eight-week-old quail using 16S rRNA gene sequencing and qPCR, and evaluated the concentrations of short-chain fatty acids (SCFAs) in each individual location using gas chromatography. The results showed that Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, and Deferribacteres were the five most abundant phyla in the intestinal tract of quail. Firmicutes was largely dominant (>95%) in the small intestine, whereas Bacteroidetes increased significantly in the cecum (19.19%) and colorectum (8.09%). At the genus level,Lactobacilluswas predominant in almost all sections (>50%) except in the cecum (7.26%), whereMegamonas,Faecalibacterium, andBacteroideswere dominant. qPCR data indicated that the population sizes of both the total bacteria and proportions of the Firmicutes, Bacteroidetes, andBacteroidesgroup increased going from the proximal toward the distal end of the intestine in quail. The SCFA-producing bacterial generaBacteroides,Faecalibacterium,Alistipes,Blautia,Parabacteroides, andClostridiumwere of higher richness in the cecum and colorectum, where, accordingly, more SCFAs were produced. These findings will be helpful for the future study of quail microbiology, as well as its relationship with productive performance and health.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序