Root-Secreted Spermine Binds to Bacillus amyloliquefaciens SQR9 Histidine Kinase KinD and Modulates Biofilm Formation

文献类型： 外文期刊

第一作者： Liu, Yunpeng

作者： Liu, Yunpeng;Xiong, Qin;Zhang, Ruifu;Feng, Haichao;Zhang, Huihui;Dong, Xiaoyan;Zhang, Ruifu;Chen, Lin

作者机构：

关键词： Bacillus amyloliquefaciens; biocontrol; genetics and gene regulation; root exudates; KinD; interaction; ligands; molecular signaling

期刊名称：MOLECULAR PLANT-MICROBE INTERACTIONS （ 影响因子：4.171； 五年影响因子：4.836 ）

ISSN： 0894-0282

年卷期： 2020 年 33 卷 3 期

页码：

收录情况： SCI

摘要： The signal molecules in root exudates that are sensed by plant growth-promoting rhizobacteria (PGPR) are critical to regulate their root colonization. Phosphorylated Spo0A is an important global transcriptional regulator that controls colonization and sporulation in Bacillus species. In this study, we found that deletion of kinD from PGPR strain Bacillus amyloliquefaciens SQR9, encoding an original phosphate donor of Spo0A, resulted in reduced biofilm formation in root exudates compared with the wild-type strain, indicating that KinD is responsible for sensing root exudates. Ligands of B. amyloliquefaciens SQR9 KinD in cucumber root exudates were determined by both the nontargeted ligand fishing method and the targeted surface plasmon resonance detection method. In total, we screened 80 compounds in root exudates for binding to KinD and found that spermine and guanosine could bind to KinD with dissociation constant values of 213 and 51 mu M, respectively. In addition, calcium L-threonate, N-acetyl-L-aspartic acid, sodium decanoic acid, and parabanic acid could also bind weakly to KinD The three-dimensional binding models were then constructed to demonstrate the interactions between the root-secreted signals and KinD It was observed that exogenous spermine reduced the wrinkles of biofilm when kinD was deleted, indicating that KinD might be involved in sensing root-secreted spermine and stabilizing biofilm in response to this negative effector. This study provided a new insight of interaction between a rhizobacterial sensor and root-secreted signals.

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