Systemic Uptake of Fluorescent Tracers by Soybean (Glycine max (L.) Merr.) Seed and Seedlings

文献类型: 外文期刊

第一作者: Wang, Zhen

作者: Wang, Zhen;Amirkhani, Masoume;Taylor, Alan G.;Wang, Zhen;Avelar, Suemar A. G.;Yang, Daibin

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关键词: fluorescent tracer; systemic uptake; soybean; in vivo imaging system (IVIS)

期刊名称:AGRICULTURE-BASEL ( 影响因子:2.925; 五年影响因子:3.044 )

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年卷期: 2020 年 10 卷 6 期

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收录情况: SCI

摘要: Systemic seed treatment uptake was investigated in seeds and seedlings using fluorescent tracers to mimic systemic agrochemicals. Soybean was used as the model as soybean has the permeable seed coat characteristic to both charged and noncharged molecules. The purpose of the paper is to (1) screen 32 fluorescent tracers and then use optimal tracers for seed and seedling uptake, (2) investigate varietal differences in seed uptake, (3) examine the distribution of tracer uptake into 14-day-old seedlings, and (4) study the relationship between seed treatment lipophilicity, measured as log P on seed and root uptake. The major chemical families that displayed both seed and seedling uptake were coumarins and xanthenes. Seed uptake of coumarin 120 ranged from 1.1% to 4.8% of the applied seed treatment tracer from 15 yellow-seeded varieties. Rhodamine B, a xanthene compound uptake in seedlings, showed translocation from the applied seed treatment to all seedling tissues. Most of the tracer was measured in the hypocotyl and root, with lesser amounts in the epicotyl and true leaves. Log P is well documented in the literature to model systemic uptake by roots, but log P of the tracers were not related to seed uptake.

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