Development of High-Density Genetic Map by Specific-Locus Amplified Fragment (SLAF) Sequencing and Identification of QTLs Governing Flowering and Bolting Time in Chinese Kale

文献类型： 外文期刊

第一作者： Li, Guihua

作者： Li, Guihua;Guo, Juxian;Luo, Wenlong;Akram, Waheed;Ahmad, Aqeel;Fu, Mei;Anjum, Tehmina;Ali, Basharat;Adnan, Muhammad

作者机构：

关键词： Bolting time; Chinese kale; Flowering time; Genetic map; SLAF-seq

期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）

ISSN： 1560-8530

年卷期： 2020 年 24 卷 3 期

页码：

收录情况： SCI

摘要： This study was aimed on finding quantitative trait loci (QTL) controlling flowering (FT) and bolting (BT) time traits in Chinese kale (Brassica oleracea var. Alboglabra) along with the candidate gene analysis. Firstly, a genetic map of high density was created by Specific-locus amplified fragment (SLAF) sequencing technique using an F-2 population of 159 individuals of B. oleracea var. Alboglabra cultivars 'Dasun' and 'LBO7M' with varying flowering times. The genetic map contained 4,296 loci, 8,500 SNPs spanning 1,075.66 cM over 9 chromosomes. The linkage analysis fine mapped the loci controlling BT and FT within 0.315 and 0.314 cM, respectively. Quantitative trait loci (QTLs) analysis showed a locus on Chr 2 governing bolting time (BT) and flowering time (FT) traits, explaining 41.14 and 48.08% of phenotypic variation (PVE), respectively along with one minor QTL of BT explaining 15% of PVE on chromosome 7. The QTLs were aligned with the physical map positions of a B. oleracea reference genome. The candidate genes were preliminary annotated based on homology, located within the QTLs regions for the BT and FT traits. Regarding BT trait, two candidate genes were identified as Bo2g024555 and Bo2g009900, which control shoot apical and floral meristems, respectively. But, the gene Bo2g048220, which controls gibberellic acid production, was selected as being putatively responsible for the FT trait. In conclusion, the QTLs found in this study provide information for marker-assisted selection and could contribute to develop early maturing Chinese kale varieties. (C) 2020 Friends Science Publishers

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