qRf8-1, a Novel QTL for the Fertility Restoration of Maize CMS-C Identified by QTL-seq

文献类型： 外文期刊

第一作者： Zheng, Mingmin

作者： Zheng, Mingmin;Yang, Tian;Liu, Xiaowei;Zhang, Peng;Jiang, Bin;Zhou, Shufeng;Lu, Yanli;Lan, Hai;Zhang, Suzhi;Li, Chuan;Rong, Tingzhao;Cao, Moju;Lu, Guihua

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关键词： Maize; Cytoplasmic male sterility; Fertility restoration; Restorer gene; QTL-seq

期刊名称：G3-GENES GENOMES GENETICS （ 影响因子：3.154； 五年影响因子：3.369 ）

ISSN： 2160-1836

年卷期： 2020 年 10 卷 7 期

页码：

收录情况： SCI

摘要： C-type cytoplasmic male sterility (CMS-C), one of the three major CMS types in maize, has a promising application prospect in hybrid seed production. However, the complex genetic mechanism underlying the fertility restoration of CMS-C remains poorly understood. The maize inbred line A619 is one of the rare strong restorer lines carrying the restorer geneRf4, but different fertility segregation ratios are found in several F(2)populations derived from crosses between isocytoplasmic allonucleus CMS-C lines and A619. In the present study, the segregation ratios of fertile to sterile plants in the (CHuangzaosi x A619) F(2)and BC(1)F(1)populations (36.77:1 and 2.36:1, respectively) did not follow a typical monogenic model of inheritance, which suggested that some F(2)and BC(1)F(1)plants displayed restored fertility even withoutRf4. To determine the hidden locus affecting fertility restoration, next-generation sequencing-based QTL-seq was performed with two specific extreme bulks consisting of 30 fertile and 30 sterilerf4rf4individuals from the F(2)population. A major QTL related to fertility restoration, designatedqRf8-1, was detected on the long arm of chromosome 8 in A619. Subsequently,qRf8-1was further validated and narrowed down to a 17.93-Mb genomic interval by insertion and deletion (InDel) and simple sequence repeat (SSR) marker-based traditional QTL mapping, explaining 12.59% (LOD = 25.06) of the phenotypic variation. Thus, using genetic analyses and molecular markers, we revealed another fertility restoration system acting in parallel withRf4in A619 that could rescue the male sterility of CHuangzaosi. This study not only expands the original fertility restoration system but also provides valuable insights into the complex genetic mechanisms underlying the fertility restoration of CMS-C.

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