文献类型： 外文期刊

第一作者： Zhang, Meng

作者： Zhang, Meng;Lai, Yiwei;Guo, Pengcheng;Guo, Xiangpeng;Zhou, Jianguo;Xu, Yan;Yu, Zhijun;Liu, Longqi;Li, Na;Lv, Yuan;Jiang, Mengling;Tariq, Muqddas;Kanwal, Shahzina;Liu, Hao;Chen, Shuhan;Wang, Dongye;Ward, Carl;Ibanez, David P.;Li, Yunpan;Bao, Xichen;Volpe, Giacomo;Esteban, Miguel A.;Zhang, Meng;Lai, Yiwei;Guo, Pengcheng;Guo, Xiangpeng;Zhou, Jianguo;Xu, Yan;Yu, Zhijun;Liu, Longqi;Ma, Gang;Li, Na;Lv, Yuan;Jiang, Mengling;Tariq, Muqddas;Kanwal, Shahzina;Liu, Hao;Xu, Xueting;Zhang, Hui;Huang, Yinghua;Wang, Lulu;Chen, Shuhan;Wang, Dongye;Ward, Carl;Ibanez, David P.;Li, Yunpan;Bao, Xichen;Qin, Baoming;Cosma, Maria Pia;Volpe, Giacomo;Esteban, Miguel A.;Zhang, Meng;Lai, Yiwei;Guo, Pengcheng;Guo, Xiangpeng;Zhou, Jianguo;Xu, Yan;Yu, Zhijun;Liu, Longqi;Ma, Gang;Li, Na;Lv, Yuan;Jiang, Mengling;Tariq, Muqddas;Kanwal, Shahzina;Liu, Hao;Xu, Xueting;Zhang, Hui;Huang, Yinghua;Wang, Lulu;Chen, Shuhan;Wang, Dongye;Ward, Carl;Ibanez, David P.;Li, Yunpan;Bao, Xichen;Qin, Baoming;Cosma, Maria Pia;Volpe, Giacomo;Esteban, Miguel A.;Zhang, Meng;Lai, Yiwei;Lv, Yuan;Tariq, Muqddas;Wang, Lulu;Chen, Shuhan;Ibanez, David P.;Qin, Yan;Zhang, Meng;Lai, Yiwei;Guo, Xiangpeng;Li, Wenjuan;Zhang, Hui;Huang, Yinghua;Wang, Lulu;Luo, Zhiwei;Ward, Carl;Bao, Xichen;Wu, Guangming;Qin, Baoming;Cosma, Maria Pia;Volpe, Giacomo;Esteban, Miguel A.;Zhang, Meng;Lai, Yiwei;Guo, Pengcheng;Guo, Xiangpeng;Zhou, Jianguo;Li, Wenjuan;Li, Na;Lv, Yuan;Jiang, Mengling;Tariq, Muqddas;Kanwal, Shahzina;Liu, Hao;Chen, Shuhan;Luo, Zhiwei;Wang, Dongye;Ward, Carl;Ibanez, David P.;Li, Yunpan;Qin, Baoming;Volpe, Giacomo;Esteban, Miguel A.;Zhang, Meng;Lai, Yiwei;Guo, Pengcheng;Guo, Xiangpeng;Zhou, Jianguo;Li, Wenjuan;Li, Na;Lv, Yuan;Jiang, Mengling;Tariq, Muqddas;Kanwal, Shahzina;Liu, Hao;Chen, Shuhan;Luo, Zhiwei;Wang, Dongye;Ward, Carl;Ibanez, David P.;Li, Yunpan;Qin, Baoming;Volpe, Giacomo;Esteban, Miguel A.;Krupalnik, Vladislav;Hanna, Jacob H.;Guo, Pengcheng;Jiang, Ao;Chen, Liang;Li, Wenjuan;Luo, Zhiwei;Ma, Gang;Xu, Xueting;Zhang, Hui;Huang, Yinghua;Wang, Lulu;Qin, Baoming;Fu, Xiuling;Babarinde, Isaac A.;Hutchins, Andrew P.;Zhou, Tiantian;Xu, Guo-Liang;He, Minghui;Zhou, Jiajian;Yuan, Jie;Sun, Hao;Feng, Yayan;Gao, Fei;Arumugam, Karthik;Di Vicino, Umberto;Cosma, Maria Pia;Arumugam, Karthik;Di Vicino, Umberto;Cosma, Maria Pia;Schambach, Axel;Schambach, Axel;Wang, Huating;Gao, Fei;Doble, Bradley W.;Doble, Bradley W.;Hartmann, Christine;Cosma, Maria Pia;Qin, Yan;Chen, Runsheng;Qin, Yan;Xu, Guo-Liang;Esteban, Miguel A.

作者机构：

期刊名称：SCIENCE ADVANCES （ 影响因子：14.136； 五年影响因子：16.446 ）

ISSN： 2375-2548

年卷期： 2020 年 6 卷 29 期

页码：

收录情况： SCI

摘要： Mouse embryonic stem cells cultured with MEK (mitogen-activated protein kinase kinase) and GSK3 (glycogen synthase kinase 3) inhibitors (2i) more closely resemble the inner cell mass of preimplantation blastocysts than those cultured with SL [serum/leukemia inhibitory factor (LIF)]. The transcriptional mechanisms governing this pluripotent ground state are unresolved. Release of promoter-proximal paused RNA polymerase II (Pol2) is a multistep process necessary for pluripotency and cell cycle gene transcription in SL. We show that B-catenin, stabilized by GSK3 inhibition in medium with 2i, supplies transcriptional coregulators at pluripotency loci. This selectively strengthens pluripotency loci and renders them addicted to transcription initiation for productive gene body elongation in detriment to Pol2 pause release. By contrast, cell cycle genes are not bound by beta-catenin, and proliferation/self-renewal remains tightly controlled by Pol2 pause release under 2i conditions. Our findings explain how pluripotency is reinforced in the ground state and also provide a general model for transcriptional resilience/adaptation upon network perturbation in other contexts.

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