Whole-genome sequencing and genomic-based acid tolerance mechanisms ofLactobacillus delbrueckiisubsp.bulgaricusLJJ

文献类型： 外文期刊

第一作者： Li, Weixun

作者： Li, Weixun;Yang, Lan;Lu, Jing;Zhang, Shuwen;Ujiroghene, Obaroakpo Joy;Pang, Xiaoyang;Lv, Jiaping;Nan, Wenlong;Ujiroghene, Obaroakpo Joy

作者机构：

关键词： Lactobacillus delbrueckiisubsp; bulgaricus; Association analysis; RT-qPCR; dapA; dapH; lysC; Acid-tolerant mechanism; The synthesis of lysine

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2020 年 104 卷 17 期

页码：

收录情况： SCI

摘要： The probiotic efficacy and fermentative ability ofLactobacillus delbrueckiisubsp.bulgaricus(L. bulgaricus), a widely used probiotic, is majorly affected by its acid tolerance. Here, we conducted whole-genome sequencing of the high acid-tolerantL. bulgaricusLJJ stored in the laboratory. Compared with the whole genome of low acid-tolerant strainL. bulgaricusATCC11842, the results show that 16 candidate acid-tolerant genes may be involved in the regulation of the acid tolerance ofL. bulgaricusLJJ. Association analysis of candidate acid-tolerant genes and acid-tolerant traits of differentL. bulgaricusstrains revealed that the three genesdapA,dapH, andlysCare the main reasons for the strong acid tolerance ofL. bulgaricusLJJ. The results of real-time quantitative PCR (RT-qPCR) supported this conclusion. KEGG pathway analysis showed that these three acid-tolerant genes are involved in the synthesis of lysine; the synthesis of lysine may conferL. bulgaricusLJJ strong acid tolerance. This study successfully revealed the acid tolerance mechanism ofL. bulgaricusLJJ and provides a theoretical basis for the subsequent selection of strains with high acid tolerance for improved probiotic functions.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序