Identification of the vernalization geneVRN-B1responsible for heading date variation by QTL mapping using a RIL population in wheat

文献类型： 外文期刊

第一作者： Li, Yuting

作者： Li, Yuting;Xiong, Hongchun;Guo, Huijun;Zhou, Chunyun;Xie, Yongdun;Zhao, Linshu;Gu, Jiayu;Zhao, Shirong;Ding, Yuping;Liu, Luxiang

作者机构：

关键词： Heading time; Wheat; BSR-Seq; Vrn-B1; Genetic mapping; Metabolic pathways

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2020 年 20 卷 1 期

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收录情况： SCI

摘要： Background Heading time is one of the most important agronomic traits in wheat, as it largely affects both adaptation to different agro-ecological conditions and yield potential. Identification of genes underlying the regulation of wheat heading and the development of diagnostic markers could facilitate our understanding of genetic control of this process. Results In this study, we developed 400 recombinant inbred lines (RILs) by crossing a gamma-ray-induced early heading mutant (eh1) with the late heading cultivar, Lunxuan987. Bulked Segregant Analysis (BSA) of both RNA and DNA pools consisting of various RILs detected a quantitative trait loci (QTL) for heading date located on chromosomes 5B, and further genetic linkage analysis limited the QTL to a 3.31 cM region. We then identified a large deletion in the first intron of the vernalization geneVRN-B1ineh1,and showed it was associated with the heading phenotype in the RIL population. However, it is not the mutation loci that resulted in early heading phonotype in the mutant compared to that of wildtype. RNA-seq analysis suggested thatVrn-B3and several newly discovered genes, including beta-amylase 1 (BMY1) and anther-specific protein (RTS), were highly expressed in both the mutant and early heading pool with the dominantVrn-B1genotype compared to that of Lunxuan987 and late heading pool. Enrichment analysis of differentially expressed genes (DEGs) identified several key pathways previously reported to be associated with flowering, including fatty acid elongation, starch and sucrose metabolism, and flavonoid biosynthesis. Conclusion The development of new markers forVrn-B1in this study supplies an alternative solution for marker-assisted breeding to optimize heading time in wheat and the DEGs analysis provides basic information forVRN-B1regulation study.

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