Purple acid phosphatase 10c encodes a major acid phosphatase that regulates plant growth under phosphate-deficient conditions in rice

文献类型： 外文期刊

第一作者： Deng, Suren

作者： Deng, Suren;Li, Jingyi;Du, Zezhen;Liu, Tongtong;Li, Wenjing;Xu, Fangsen;Shi, Lei;Wang, Chuang;Deng, Suren;Li, Jingyi;Du, Zezhen;Liu, Tongtong;Li, Wenjing;Xu, Fangsen;Shi, Lei;Wang, Chuang;Lu, Linghong;Shou, Huixia;Wang, Chuang

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关键词： Acid phosphatase; native promoter; organic phosphate; overexpression; Pi deficiency; rice; root

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）

ISSN： 0022-0957

年卷期： 2020 年 71 卷 14 期

页码：

收录情况： SCI

摘要： Whilst constitutive overexpression of particular acid phosphatases (APases) can increase utilization of extracellular organic phosphate, negative effects are frequently observed in these transgenic plants under conditions of inorganic phosphate (Pi) sufficiency. In this study, we identified rice purple acid phosphatase 10c (OsPAP10c) as being a novel and major APase that exhibits activities associated both with the root surface and with secretion. Two constructs were used to generate the OsPAP10c-overexpression plants by driving its coding sequence with either a ubiquitin promoter (UP) or the OsPAP10c-native promoter (NP). Compared with the UP transgenic plants, lower expression levels and APase activities were observed in the NP plants. However, the UP and NP plants both showed a similar ability to degrade extracellular ATP and both promoted root growth. The growth performance and yield of the NP transgenic plants were better than the wild-type and UP plants in both hydroponic and field experiments irrespective of the level of Pi supply. Overexpression of APase by its native promoter therefore provides a potential way to improve crop production that might avoid increased APase activity in untargeted tissues and its inhibition of the growth of transgenic plants.

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