Bioactivities and Molecular Characterizations of the Polysaccharide(s) from Ultrasonic-Circulating Extracts of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes)

文献类型： 外文期刊

第一作者： Chen, Ti-Qiang

作者： Chen, Ti-Qiang;Yang, Chi;Kan, Yong -Jun;Wu, Jian-Guo;Wu, Jin-Zhong

作者机构：

关键词： Ganoderma lucidum; polysaccharide; ultrasonic-circulating extraction; ethanol fractionation; antioxidant activity; hepatoprotective activity; medicinal mushrooms

期刊名称：INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS （ 影响因子：1.921； 五年影响因子：1.879 ）

ISSN： 1521-9437

年卷期： 2020 年 22 卷 5 期

页码：

收录情况： SCI

摘要： Total polysaccharide (i.e., GLP-UCE) was effectively isolated from the crude polysaccharide extract powder of Ganoderma lucidum by ultrasonic-circulating extraction, which was separated into three fractions (i.e., GLP-U40, GLP-U60, and GLP-U80) by ethanol fractional precipitation. The detection of antioxidant enzymes (GSH-Px, SOD) and oxidation metabolites (MDA, LF); liver function test of ALT, AST, and LDH; and western blot for antioxidant proteins of GSH-Px and MMP-2 showed that the GLP-UCE displayed a favorable in vivo antioxidant and hepatoprotective activities for reducing the oxidative damage in CCl4-hepatopathy SD rats. The GLP-UCE and its fractions were analyzed and compared by Fourier transform infrared (FT-IR), high-performance liquid chromatography, high-performance gel permeation chromatography, and antioxidant activity in vitro assay. These studies revealed that the fraction GLP-U80 exhibited stronger antioxidant activities in vitro than that of GLP-UCE and other fractions (p < 0.01), which was homogeneous and heteropolysaccharide-rich in beta-D-glucose with a weight-average molecular mass (M-w) of 1.05 x 10(4) g.mol(-1) and composed of D-mannose, D-ribose, D-glucose, D-galactose, and L-fucose in a molecular ratio of 1:0.26:8.26:0.64:0.08.

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