Baseline susceptibility and resistance allele frequency inOstrinia furnacalisrelated to Cry1 toxins in the Huanghuaihai summer corn region of China

文献类型： 外文期刊

第一作者： Li, Guoping

作者： Li, Guoping;Huang, Jianrong;Ji, Tingjie;Tian, Caihong;Feng, Hongqiang;Zhao, Xincheng

作者机构：

关键词： Ostrinia furnacalis; susceptibility; diagnostic concentrations; resistance allele frequency

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期： 2020 年 76 卷 12 期

页码：

收录情况： SCI

摘要： BACKGROUND Ostrinia furnacalis(Guenee) is one of the most destructive pests of corn and is a major target of transgenic corn expressing Bt (Bacillus thuringiensis) toxins in the Huanghuaihai summer corn region of China. Prior to the widespread commercialization of transgenic Bt corn, it is necessary to estimate baseline susceptibility to Bt toxins and Bt toxin resistance allele frequencies inO. furnacalis. RESULTS The median lethal concentration (LC50) values of the Bt toxins Cry1Ab, Cry1Ac and Cry1F for 15 different populations ranged from 0.887 to 1.617, 1.251 to 2.594 and 4.146 to 6.465 ng cm(-2), respectively. The LC(99)values of 93, 45, and 197 ng cm(-2)for Cry1Ab, Cry1Ac and Cry1F, respectively, killed > 99% of individuals of eightO. furnacalispopulations collected in 2017 and were identified as diagnostic concentrations for monitoring susceptibility inO. furnacalispopulations in this region. Using the F(2)screening method with these diagnostic concentrations, the resistance allele frequencies related to Cry1Ab, Cry1Ac and Cry1F were found to be 0.002 (0.000283-0.006484), 0.001 (0.000030-0.004295) and 0.001 (0.000030-0.004295), respectively, in 2018. CONCLUSION Fifteen populations ofO. furnacaliscollected in the Huanghuaihai summer corn region of China were all susceptible to Cry1Ab, Cry1Ac and Cry1F toxins, and the susceptibility showed no significant variation among theseO. furnacalispopulations. The estimated resistance allele frequency to Cry1Ab, Cry1Ac and Cry1F was rare in this region. This provided essential knowledge for making the decision to commercialize Bt maize, and monitoring resistance development and evaluating resistance management strategies in the future in the Huanghuaihai summer corn region of China.

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