Characterization of Est-SSR markers related toColletotrichum fructicolainfection in strawberry (FragariaxananassaDuchase)

文献类型： 外文期刊

第一作者： Zou, Xiaohua

作者： Zou, Xiaohua;Huang, Xilun;Zhang, Liqing;Gao, Qing-Hua

作者机构：

关键词： strawberry; EST-SSR; polymorphism; resistance; transcriptome

期刊名称：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT （ 影响因子：1.632； 五年影响因子：2.029 ）

ISSN： 1310-2818

年卷期： 2020 年 34 卷 1 期

页码：

收录情况： SCI

摘要： Colletotrichum fructicolacauses strawberry anthracnose, which results in considerable economic losses in quality and production in Asia. However, the molecular mechanism of strawberry infection withC. fructicolais still poorly understood. Here, we employed RNA-Seq technology to mine the strawberry transcriptome for EST-SSRs (Expressed Sequence Tag-Simple Sequence Repeats) followingC. fructicolainfection. Fortunately, we detected 3019 potential EST-SSRs from the transcriptomic data of cv. Jiuxiang. The seven commonly occurring repeat motifs were A/T (278), GAA/TTC (263), GAG/CTC (215), AAG/CTT(188), GGA/TCC (163), AGA/TCT (153) and AGG/CCT (153). We identified 31, 84 and 129 differentially expressed EST-SSR-containing sequences corresponding to 24, 72 and 96 Hpi, while 13 DE-EST-SSRs -containing sequences were differentially expressed and common for all the three time points. Furthermore, we identified allele polymorphisms for some DE-EST-SSRs among several resistant and susceptible strawberry varieties and wild species. The EST-SSRs in our research were related to the reported QTL resistance loci in strawberry which are differentially expressed afterC. fructicolainfection and have allele polymorphisms in different strawberry varieties and wild species. These provide a clue for utilisation of the EST-SSRs in marker-assisted breeding to improve anthracnose-resistant breeding in future.

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