Vaccination of Mice withListeria ivanoviiExpressing the Truncated M Protein of Porcine Reproductive and Respiratory Syndrome Virus Induces both Antigen-Specific CD4+and CD8+T Cell-Mediated Immunity

文献类型： 外文期刊

第一作者： Tang, Tian

作者： Tang, Tian;Wang, Chuan;Pu, Qikang;Liu, Sijing;Ren, Chenyan;Jiang, Mingjuan;Peng, Jinmei;Tian, Zhijun

作者机构：

关键词： Porcine reproductive and respiratory syndrome virus; Listeria; Vaccine

期刊名称：JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：0.803； 五年影响因子：1.609 ）

ISSN： 1464-1801

年卷期： 2020 年 29 卷 1-6 期

页码：

收录情况： SCI

摘要： Porcine reproductive and respiratory syndrome (PRRS), a serious disease of swine caused by the PRRS virus (PRRSV), had a severe economic impact worldwide. As commonly used PRRS vaccines, the attenuated or inactivated vaccines, provide unsatisfactory immune protection, a new PRRS vaccine is urgently needed. In this study, a part of the PRRSVORF6gene (from 253 to 519 bp) encoding the hydrophilic domain of PRRSV M protein was integrated into twoListeriastrains via homologous recombination to generate two PRRS vaccine candidates, namely LI-M' and LM-Delta actAplcB-M'. Both candidate vaccines showed similar growth rate as their parent strains in culture media, but presented different bacterial loads in target organs. As the integrated heterogenous gene was not expressed, LM-Delta actAplcB-M' was excluded from the immunological test. In a mouse model, LI-M' provoked both CD4+ and CD8+ T cell-mediated immunity. In addition, LI-M' boosting dramatically enhanced CD8+ T cell-mediated immunity without affecting the response intensity of CD4+ T cell-mediated immunity. All of these data suggest that LI-M' is a promising PRRS vaccine candidate.

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