Development of a Low-Cost and High-Efficiency Culture Medium for Bacteriocin Lac-B23 Production byLactobacillus plantarumJ23

文献类型： 外文期刊

第一作者： Zhang, Jianming

作者： Zhang, Jianming;Zhang, Chengcheng;Liu, Daqun;Bu, Yushan;Yi, Huaxi;Jiao, Jingkai

作者机构：

关键词： bacteriocin Lac-B23; low-cost; high-efficiency; culture medium

期刊名称：BIOLOGY-BASEL （ 影响因子：5.079； ）

ISSN：

年卷期： 2020 年 9 卷 7 期

页码：

收录情况： SCI

摘要： At present, De Man, Rogosa and Sharpe (MRS) broth is the medium of choice for promoting bacteriocin production. However, this medium is expensive and not applicable for large-scale production. Therefore, a low-cost and high-efficiency culture medium for bacteriocin Lac-B23 production byLactobacillus plantarumJ23 was developed. First, the effects of the composition of MRS broth on bacteriocin Lac-B23 production and bacterial growth were researched by a one variable at a time approach. Then, a Plackett-Burman design was used to screen significant components for production. Finally, the steepest ascent and central composite designs were used to obtain an optimum medium. The final composition of the modified MRS was much simpler than MRS broth, and the modified MRS contained only glucose, yeast extract, dipotassium phosphate, manganese sulfate monohydrate, Tween 80 and sodium acetate anhydrous. The highest bacteriocin Lac-B23 production reached 2560 activity units (AU)/mL in the modified MRS, which is nine times higher than that in MRS broth (280 AU/mL). Meanwhile, the cost per liter of the modified MRS (8.56 Ren Min Bi (RMB)/L) is 34.70% the cost of MRS broth (13.11 RMB/L), and the cost per arbitrary units of bacteriocin Lac-B23 in the modified MRS is approximately fourteen times more convenient (3.34 RMB/10(6)AU) than in the MRS broth (46.82 RMB/10(6)AU).

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序