Identification and analysis of new mycoviruses from melon powdery mildew

文献类型： 外文期刊

第一作者： Maimaiti, Yushanjiang

作者： Maimaiti, Yushanjiang;Yang, Du;Han, Sheng;Ding, Lei;Chai, Min;Jing, Xiaoya;Sun, Liuqing;Chen, Wei;Ding, Lei;Ding, Lei

作者机构：

关键词： Mycoviruses; Podosphaera xanthii; Genetic diversity; Biological control

期刊名称：JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.729； 五年影响因子：1.681 ）

ISSN： 1125-4653

年卷期： 2020 年 102 卷 4 期

页码：

收录情况： SCI

摘要： Melon powdery mildew is a devastating disease caused byPodosphaera xanthii.To identify and characterize mycoviruses ofP. xanthii, total RNA ofP. xanthiwas extracted for constructing a library which was sequenced in a paired-end 2 x 75 bp mode of a NextSeq500 sequencer. Sequence analyses showed 87,607,242 clean reads, and 1076 unigenes were successfully matched with 151 mycovirus isolates within 71 mycovirus species. Among these isolates, 17 new isolates from four mycovirus species were identified. Their amino acid identity ranged from 32.8% to 100%. Phylogenetic relationships based on the RNA-dependent RNA polymerase protein of 37 isolates, including 20 retrieved from databases, showed a high divergence, which was confirmed by differences in genome structure and size. These results expand our understanding of the diversity of mycoviruses and will be useful for the development of potential biological control methods.

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