A cysteine protease ofBabesia microtiand its interaction with tick cystatins

文献类型： 外文期刊

第一作者： Wei, Nana

作者： Wei, Nana;Du, Yanfang;Lu, Jinmiao;Zhou, Yongzhi;Cao, Jie;Zhang, Houshuang;Gong, Haiyan;Zhou, Jinlin

作者机构：

关键词： Babesia microti; Cysteine protease; BmCYP; Tick cystatin

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.289； 五年影响因子：2.403 ）

ISSN： 0932-0113

年卷期： 2020 年 119 卷 9 期

页码：

收录情况： SCI

摘要： Babesiosis is a tick-borne protozoonosis caused byBabesia, which can cause fever, hemolytic anemia, hemoglobinuria, and even death.Babesia microtiis a parasite found in rodents and can be pathogenic to humans. In this study, the full-length cDNA of aB. microticysteine protease (BmCYP) was expressed and the recombinant rBmCYP protein analyzed and characterized. BmCYP is encoded by an ORF of 1.3 kb, with a predicted molecular weight of 50 kDa and a theoretical pI of 8.5. The amino acid sequence of BmCYP exhibits an identity of 32.9 to 35.2% with cysteine proteases ofBabesia ovis,Babesia bovis, andTheileria, respectively. The results of the proteinase assays show that rBmCYP has cysteine protease enzymatic activity. In addition, we demonstrate that tick cystatins rRhcyst-1 and rRhcyst-2 were able to effectively inhibit the activity of rBmCYP; the inhibition rates were 57.2% and 30.9%, respectively. Tick cystatinsRhcyst-1andRhcyst-2were differentially expressed in ticks that fed onBabesia-infected mice relative to non-infected control ticks. Our results suggest that BmCYP is a functional enzyme with cysteine protease enzymatic activity and may be involved in tick-B. microtiinteractions.

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