Identification and Fine Mapping ofRppM, a Southern Corn Rust Resistance Gene in Maize

文献类型： 外文期刊

第一作者： Wang, Shuai

作者： Wang, Shuai;Zhang, Ruyang;Shi, Zi;Zhao, Yanxin;Su, Aiguo;Wang, Yuandong;Xing, Jinfeng;Ge, Jianrong;Li, Chunhui;Wang, Xiaqing;Wang, Jidong;Sun, Xuan;Liu, Qian;Chen, Yining;Zhang, Yunxia;Wang, Shuaishuai;Song, Wei;Zhao, Jiuran

作者机构：

关键词： maize; southern corn rust; fine mapping; resistance gene; Puccinia polysora

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： Southern corn rust (SCR) caused byPuccinia polysoraUnderw. is a major disease causing severe yield losses during maize production. Here, we identified and mapped the SCR resistance geneRppMfrom the near-isogenic line Kangxiujing2416 (Jing2416K), which harborsRppMin the genetic background of the susceptible inbred line Jing2416. In this study, the inheritance of SCR resistance was investigated in F(2)and F(3)populations derived from a cross between Jing2416K and Jing2416. The observed 3:1 segregation ratio of resistant to susceptible plants indicated that the SCR resistance is controlled by a single dominant gene. Using an F(2)population, we performed bulked segregant analysis (BSA) sequencing and mappedRppMto a 3.69-Mb region on chromosome arm 10S. To further narrow down the region harboringRppM, we developed 13 insertion/deletion (InDel) markers based on the sequencing data. Finally,RppMwas mapped to a region spanning 110-kb using susceptible individuals from a large F(2)population. Two genes (Zm00001d023265andZm00001d023267) encoding putative CC-NBS-LRR (coiled-coiled, nucleotide-binding site, and leucine-rich repeat) proteins, a common characteristic of R genes, were located in this region (B73 RefGen_v4 reference genome). Sequencing and comparison of the two genes cloned from Jing2416K and Jing2416 revealed sequence variations in their coding regions. The relative expression levels of these two genes in Jing2416K were found to be significantly higher than those in Jing2416.Zm00001d023265andZm00001d023267are thus potentialRppMcandidates.

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