Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry-Based Metabolomics Reveals Metabolic Alterations in the Mouse Cerebellum DuringToxoplasma gondiiInfection

文献类型： 外文期刊

第一作者： Ma, Jun

作者： Ma, Jun;He, Jun-Jun;Hou, Jun-Ling;Zhu, Xing-Quan;Zhou, Chun-Xue;Elsheikha, Hany M.

作者机构：

关键词： cerebellum; Toxoplasma gondii; metabolomics; pathway analysis; host-parasite interaction

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2020 年 11 卷

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收录情况： SCI

摘要： Toxoplasma gondiiis a protozoan parasite with a remarkable neurotropism. We recently showed thatT. gondiiinfection can alter the global metabolism of the cerebral cortex of mice. However, the impact ofT. gondiiinfection on the metabolism of the cerebellum remains unknown. Here we apply metabolomic profiling to discover metabolic changes associated withT. gondiiinfection of the mouse cerebellum using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Multivariate statistics revealed differences in the metabolic profiles between the infected and control mouse groups and between the infected mouse groups as infection advanced. We also detected 10, 22, and 42 significantly altered metabolites (SAMs) in the infected cerebellum at 7, 14, and 21 days post infection (dpi), respectively. Four metabolites [tabersonine, arachidonic acid (AA), docosahexaenoic acid, and oleic acid] were identified as potential biomarker or responsive metabolites toT. gondiiinfection in the mouse cerebellum. Three of these metabolites (AA, docosahexaenoic acid, and oleic acid) play roles in the regulation of host behavior and immune response. Pathway analysis showed thatT. gondiiinfection of the cerebellum involves reprogramming of amino acid and lipid metabolism. These results showcase temporal metabolomic changes during cerebellar infection byT. gondiiin mice. The study provides new insight into the neuropathogenesis ofT. gondiiinfection and reveals new metabolites and pathways that mediate the interplay betweenT. gondiiand the mouse cerebellum.

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