Morphological, Molecular Identification and Distribution of Trypanosome-Transmitting Dipterans from Cattle Settlements in Southwest Nigeria

文献类型： 外文期刊

第一作者： Odeniran, Paul Olalekan

作者： Odeniran, Paul Olalekan;Ademola, Isaiah Oluwafemi;Odeniran, Paul Olalekan;Macleod, Ewan Thomas;Majekodunmi, Ayodele Oluwakemi;Welburn, Susan Christina;Ohiolei, John Asekhaen;Majekodunmi, Ayodele Oluwakemi;Welburn, Susan Christina

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关键词： Tsetse flies; Horse fly; Stable fly; Morphology; Phylogenetics; Southwest nigeria

期刊名称：ACTA PARASITOLOGICA （ 影响因子：1.44； 五年影响因子：1.335 ）

ISSN： 1230-2821

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收录情况： SCI

摘要： Introduction Glossinaspp. (Glossinidae),Tabanusspp. (Tabanidae),Ancalaspp. (Tabanidae),Atylotusspp. (Tabanidae) andStomoxysspp. (Muscidae) are important transmitting vectors of African animal trypanosomosis in sub-Saharan Africa. There is paucity of information on the distribution and identification of these flies in cattle settlements in southwest Nigeria. Methods The distribution patterns, genetic variations and diversities of dipteran flies in southwest Nigeria were described and identified using morphological and molecular analysis of the28S rDNAgene. Results Of the 13,895 flies examined morphologically between April 2016 and March 2017, tabanids were identified [Tabanus(0.34%),Ancala(0.03%),Atylotus(0.01%),Haematopota(0.014%) andChrysops(0.11%)]. Two stomoxyine species were identified;Stomoxys niger nigerMacquart (45.30%) andStomoxys calcitransLinnaeus (17.29%) and twoGlossinaspp. namely;Glossina p. gambienseVanderplank, 1911 (0.46%) andGlossina tachinoidesWestwood (0.51%) were identified. The identities were further confirmed in a BLAST search using their nucleotide sequences. The median-joining network of the 28SrDNAgene sequences indicated that fly species examined were genetically distinct. The apparent density of all the trapped flies was highest at a mean temperature of 26-28 celcius, humidity > 80% and rainfall of 150-220 mm/month. The distribution of flies was observed to increase as vegetation increased in density and decreased in areas with relatively high human population density (> 100/km(2)). Conclusions The population indices of the 28SrDNAgene of the flies suggest that analysis of nuclear DNA fragments may provide more information on the molecular ecology of these flies. Characterising fly species and assessing their impact are essential in distribution and monitoring AAT spread.

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