Insect group II chitinase OfChtII promotes chitin degradation during larva-pupa molting

文献类型： 外文期刊

第一作者： Qu, Ming-Bo

作者： Qu, Ming-Bo;Sun, Shao-Peng;Liu, Yuan-Sheng;Deng, Xiao-Rui;Yang, Jun;Yang, Qing;Qu, Ming-Bo;Yang, Qing;Qu, Ming-Bo;Yang, Qing

作者机构：

关键词： chitin; chitinase; chitin binding; degradation; multiple module; synergy

期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）

ISSN： 1672-9609

年卷期：

页码：

收录情况： SCI

摘要： The insect group II chitinase (ChtII, also known as Cht10) is a unique chitinase with multiple catalytic and chitin-binding domains. It has been proven genetically to be an essential chitinase for molting. However, ChtII's role in chitin degradation during insect development remains poorly understood. Obtaining this knowledge is the key to fully understanding the chitin degradation system in insects. Here, we investigated the role of OfChtII during the molting ofOstrinia furnacalis, a model lepidopteran pest insect. OfChtII was expressed earlier than OfChtI (OfCht5) and OfChi-h, at both the gene and protein levels during larva-pupa molting as evidenced by quantitative polymerase chain reaction and western blot analyses. A truncated OfChtII, OfChtII-B4C1, was recombinantly expressed inPichia pastoriscells and purified to homogeneity. The recombinant OfChtII-B4C1 loosened compacted chitin particles and produced holes in the cuticle surface as evidenced by scanning electron microscopy. It synergized with OfChtI and OfChi-h when hydrolyzing insoluble alpha-chitin. These findings suggested an important role for ChtII during insect molting and also provided a strategy for the coordinated degradation of cuticular chitin during insect molting by ChtII, ChtI and Chi-h.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序