First Detection and Molecular Identification ofEntamoebain Yaks from China

文献类型： 外文期刊

第一作者： Ren, Mei

作者： Ren, Mei;Yang, Fan;Gou, Jing-min;Wang, Pin-xue;Zou, Min;Zhong, Xiao-hong;Lin, Qing;Ren, Mei;Lin, Qing;Ren, Mei;Lin, Qing

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关键词： Qinghai; Yak; Entamoeba; 18S rRNA; Phylogeny

期刊名称：ACTA PARASITOLOGICA （ 影响因子：1.44； 五年影响因子：1.335 ）

ISSN： 1230-2821

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收录情况： SCI

摘要： Background Yak, a predominant livestock of plateau areas, is known as a host to many parasites. And the genusEntamoeba, the third-common cause of the mortality worldwide from parasitic diseases, was discovered in yaks once. Methods We investigated the distribution and species ofEntamoebaspp. from yaks in Qinghai province, northwestern China, by collecting 1027 yak fecal samples. All samples were divided according to seven geographical sites, four seasons, and two age groups of yaks. After extracting DNA, polymerase chain reaction (PCR) was performed to amplify the 18S rRNA gene, and sequences were analyzed with phylogenetic method. Results We observed an overallEntamoebapositive rate of 36.32% (373/1027) in yaks from Qinghai province. The common species includedEntamoeba bovis(284/373),Entamoebasp. MG107/BEL (79/373),Entamoebasp. ribosomal lineage (RL) two (8/373), andEntamoebasp. RL9 (2/373).According to the result of statistical analysis,Entamoebainfection rate was the highest in summer and significantly differed from that observed during other seasons (P < 0.05). The yaks from Golog had the highest prevalence ofEntamoebaamong all geographical origins in Qinghai province (P < 0.05). However, no significant difference was observed (P > 0.05) among different age groups, as evident from a positive rate of 39.58% in <= 6-month and 36.16% in > 6-month yaks. Conclusion These results indicate the prevalence and predominant species ofEntamoebain yaks. To our knowledge, this is the first study to reportE. bovis,Entamoebasp. RL2, andEntamoebasp. RL9 in Chinese yaks.

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