HalophyticHordeum brevisubulatumHbHAK1 Facilitates Potassium Retention and Contributes to Salt Tolerance

文献类型： 外文期刊

第一作者： Zhang, Haiwen

作者： Zhang, Haiwen;Xiao, Wen;Yu, Wenwen;Jiang, Ying;Li, Ruifen;Xiao, Wen;Yu, Wenwen

作者机构：

关键词： HAK; KUP; KT transporter; K+-uptake; salt-tolerance; K+; Na(+)homeostasis; site mutation

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2020 年 21 卷 15 期

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收录情况： SCI

摘要： Potassium retention under saline conditions has emerged as an important determinant for salt tolerance in plants. HalophyticHordeum brevisubulatumevolves better strategies to retain K(+)to improve high-salt tolerance. Hence, uncovering K+-efficient uptake under salt stress is vital for understanding K(+)homeostasis. HAK/KUP/KT transporters play important roles in promoting K(+)uptake during multiple stresses. Here, we obtained nine salt-induced HAK/KUP/KT members inH. brevisubulatumwith different expression patterns compared withH. vulgarethrough transcriptomic analysis. One member HbHAK1 showed high-affinity K(+)transporter activity inathak5to cope with low-K(+)or salt stresses. The expression of HbHAK1 in yeast Cy162 strains exhibited strong activities in K(+)uptake under extremely low external K(+)conditions and reducing Na(+)toxicity to maintain the survival of yeast cells under high-salt-stress. Comparing with the sequence of barley HvHAK1, we found that C170 and R342 in a conserved domain played pivotal roles in K(+)selectivity under extremely low-K(+)conditions (10 mu M) and that A13 was responsible for the salt tolerance. Our findings revealed the mechanism of HbHAK1 for K(+)accumulation and the significant natural adaptive sites for HAK1 activity, highlighting the potential value for crops to promote K+-uptake under stresses.

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