Genome-wide identification and expression analysis of SABATH methyltransferases in tea plant (Camellia sinensis): insights into their roles in plant defense responses

文献类型： 外文期刊

第一作者： Guo, Yan

作者： Guo, Yan;Qiao, Dahe;Yang, Chun;Chen, Juan;Li, Yan;Liang, Sihui;Lin, Kaiqin;Chen, Zhengwu

作者机构：

关键词： Camellia sinensis; SABATH gene family; methyltransferase; plant defense; MeSA

期刊名称：PLANT SIGNALING & BEHAVIOR （ 影响因子：2.247； 五年影响因子：2.369 ）

ISSN： 1559-2316

年卷期： 2020 年 15 卷 10 期

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收录情况： SCI

摘要： SABATH methyltransferases convent plant small-molecule metabolites into volatile methyl esters, which play important roles in many biological processes and defense reactions in plants. In this study, a total of 32SABATHgenes were identified in theCamellia sinensisvar.sinensis(CSS) genome, which were renamed CsSABATH1 to CsSABATH32. Genome location annotation suggested that tandem duplication was responsible for the expansion ofSABATHgenes in tea plant. Multiple sequence alignment and phylogenetic analysis showed that the CsSABATHs could be classified into three groups (I, II and III), which were also supported by gene structures and conserved motifs analysis. Group II contained only two CsSABATH proteins, which were closely related to PtIAMT, AtIAMT and OsIAMT. The group III SABATH genes of tea plant exhibited expansion on the CSS genome compared withCamellia sinensisvar.assamica(CSA) genome. Based on RNA-seq data, theCsSABATHsexhibited tissue-specific expression patterns, and the members with high expression in buds and young leaves were also obviously upregulated after MeJA treatment. The expression of many transcription factors was significantly correlated with that of different members of theCsSABATHgene family, suggesting a potential regulatory relationship between them. Quantitative real-time PCR (qPCR) expression analysis showed thatCsSABATHscould respond to exogenous JA, SA and MeSA treatments in tea plants. RNA-seq data analysis and qPCR validation suggested thatCsSABATH8, 11, 16, 25, 29 and 32 might play a special role in plant defense against insect herbivory. These results provide references for evolutionary studies of the plantSABATHfamily and the exploration of the potential roles ofCsSABATHsin tea plant defense responses.

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