A Fragment Substitution in Promoter of MS92/PTC1 Causes Male Sterility in Rice

文献类型： 外文期刊

第一作者： Peng, Qin

作者： Peng, Qin;Luchang, Deng;Weilan, Chen;Shijun, Fan;Bin, Tu;Hua, Yuan;Yuping, Wang;Bingtian, Ma;Shigui, Li;Luchang, Deng;Jun, Tan;Juan, Huang

作者机构：

关键词： rice; MS92/PTC1; plant homeodomain finger; cis-element; male sterility; anther

期刊名称：RICE SCIENCE （ 影响因子：5.6； 五年影响因子：5.1 ）

ISSN： 1672-6308

年卷期： 2020 年 27 卷 5 期

页码：

收录情况： SCI

摘要： Persistent tapetal cell1 (PTC1) plays a curial role in pollen development, and is thought to function as a transcriptional activator in rice. However, the molecular mechanism of PTC1 in regulating pollen development and its cis-elements are not well understood. We identified a novel weak male sterility mutant (ms92) which exhibited expanded tapetum and shrink pollen grains. Map-based cloning and allelic analysis suggested that the male sterility of ms92 was caused by a DNA fragment substitution in the promoter of PTC1. The decreased expression of MS92/PTC1 in ms92 and cis-element analysis indicated that the substituted sequence contained several potential binding cis-element of negative feedback. MS92/PTC1 was specifically expressed in tapetum and microspores at the young microspore stage, and its protein was localized in nucleus. We further found that MS92/PTC1 functions as a transcription activator by recognizing H3K4me3. Transcriptomic analysis revealed that a number of genes involved in tapetum degeneration and pollen wall formation were down-regulated in ms92, which are the potential targets of MS92/PTC1. The substitution fragment in MS92/PTC1 promoter was essential for pollen development, and we provided a novel mutant for further identifying the cis-elements in promoter and the molecular network of MS92/PTC1.

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