UvAtg8-Mediated Autophagy Regulates Fungal Growth, Stress Responses, Conidiation, and Pathogenesis inUstilaginoidea virens

文献类型： 外文期刊

第一作者： Meng, Shuai

作者： Meng, Shuai;Jagernath, Jane Sadhna;Wang, Congcong;Qiu, Jiehua;Shi, Huanbin;Kou, Yanjun;Xiong, Meng

作者机构：

关键词： UvAtg8; Rice false smut; Ustilaginoidea virens; Autophagy; Secondary spore; Pathogenicity

期刊名称：RICE （ 影响因子：4.783； 五年影响因子：5.23 ）

ISSN： 1939-8425

年卷期： 2020 年 13 卷 1 期

页码：

收录情况： SCI

摘要： Background Ustilaginoidea virenshas become one of the most devastating rice pathogens in China, as well as other rice-growing areas. Autophagy is an important process in normal cell differentiation and development among various organisms. To date, there has been no optimized experimental system introduced for the study of autophagy inU. virens. In addition, the function of autophagy in pathogenesis remains unknown inU. virens.Therefore, the functional analyses of UvAtg8 may potentially shed some light on the regulatory mechanism and function of autophagy inU. virens. Results In this study, we characterized the functions of UvAtg8, which is a homolog ofSaccharomyces cerevisiaeScAtg8, in the rice false smut fungusU. virens. The results showed thatUvATG8is essential for autophagy inU. virens.Also, theGFP-UvATG8strain, which could serve as an appropriate marker for monitoring autophagy inU. virens,was generated.Furthermore, this study found that the Delta Uvatg8mutant was defective in the vegetative growth, conidiation, adaption to oxidative, hyperosmotic, cell wall stresses, and production of toxic compounds. Pathogenicity assays indicated that deletion ofUvATG8resulted in significant reduction in virulence ofU. virens. Further microscopic examinations of the infection processes revealed that the severe virulence defects in the increment Uvatg8were mainly caused by the highly reduced conidiation and secondary spore formation. Conclusions Our results indicated that the UvAtg8 is necessary for the fungal growth, stresses responses, conidiation, secondary spore formation, and pathogenicity ofU. virens. Moreover, our research finding will potentially assist in further clarifying the molecular mechanism ofU. virensinfection, as well as provide a good marker for autophagy inU. virensand a good reference value for the further development of effective fungicides based on gene targeting.

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