Butyrate-mediated autophagy inhibition limits cytosolicSalmonellaInfantis replication in the colon of pigs treated with a mixture ofLactobacillusandBacillus

文献类型： 外文期刊

第一作者： Chu, Bingxin

作者： Chu, Bingxin;Zhu, Yaohong;Su, Jinhui;Xia, Bing;Zou, Yunjing;Nie, Jiawei;Wang, Jiufeng;Zhang, Wei

作者机构：

关键词： Salmonella; butyrate; autophagy; microbiota; probiotic; colon

期刊名称：VETERINARY RESEARCH （ 影响因子：3.683； 五年影响因子：4.106 ）

ISSN： 0928-4249

年卷期： 2020 年 51 卷 1 期

页码：

收录情况： SCI

摘要： Probiotics as an effective and safe strategy for controllingSalmonellainfection are much sought after, while autophagy is a central issue in eliminating intracellular pathogens of intestinal epithelial cells. In this study, an animal model of colitis has been developed by infecting weaned pigs orally with a strain ofSalmonellaInfantis in order to illuminate the potential efficacy of a mixture ofLactobacillusandBacillus(CBB-MIX) in the resistance toSalmonellainfection by regulating butyrate-mediated autophagy. We found that CBB-MIX alleviatedS. Infantis-induced colitis and tissue damage. Autophagy markers ATG5, Beclin-1, and the LC3-II/I ratio were significantly enhanced byS. Infantis infection, while treatment with CBB-MIX suppressedS. Infantis-induced autophagy. Additionally,S. Infantis-induced colonic microbial dysbiosis was restored by this treatment, which also preserved the abundance of the butyrate-producing bacteria and the butyrate concentration in the colon. A Caco-2 cell model ofS. Infantis infection showed that butyrate had the same effect as the CBB-MIX in restrainingS. Infantis-induced autophagy activation. Further, the intracellularS. Infantis load assay indicated that butyrate restricted the replication of cytosolicS. Infantis rather than that inSalmonella-containing vacuoles. Suppression of autophagy by knockdown of ATG5 also attenuatedS. Infantis-induced cell injury. Moreover, hyper-replication of cytosolicS. Infantis in Caco-2 cells was significantly decreased when autophagy was inhibited. Our data demonstrated thatSalmonellamay benefit from autophagy for cytosolic replication and butyrate-mediated autophagy inhibition reduced the intracellularSalmonellaload in pigs treated with a probiotic mixture ofLactobacillusandBacillus.

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