Fine Mapping, Candidate Gene Identification and Co-segregating Marker Development for the Phytophthora Root Rot Resistance GeneRpsYD25

文献类型： 外文期刊

第一作者： Zhong, Chao

作者： Zhong, Chao;Sun, Suli;Zhang, Xuecui;Duan, Canxing;Zhu, Zhendong;Zhong, Chao

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关键词： Phytophthora root rot; fine mapping; resistance gene; RpsYD25; soybean

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）

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年卷期： 2020 年 11 卷

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收录情况： SCI

摘要： Phytophthora root rot (PRR) caused byPhytophthora sojaeis a serious disease of soybean. The most effective disease-control strategy is to deploy resistant cultivars carryingRpsgenes. Soybean cultivar Yudou25 can effectively resist pathotypes ofP. sojaein China. Previous studies have mapped theRpsgene in Yudou25,RpsYD25, on chromosome 3. In this study, at firstRpsYD25was located between SSR markers Satt1k3 (2.2 cM) and BARCSOYSSR_03_0253 (4.5 cM) by using an F(2:3)population containing 165 families derived from Zaoshu18 and Yudou25. Then the recombination sites were identified in 1127 F(3:4)families derived from Zaoshu18 and Yudou25 using the developed PCR-based SNP, InDel and SSR markers, andRpsYD25was finely mapped in the a 101.3 kb genomic region. In this region, a zinc ion binding and nucleic acid binding gene Glyma.03g034700 and two NBS-LRR genes Glyma.03g034800 and Glyma.03g034900 were predicted as candidate genes ofRpsYD25, and five co-segregated SSR markers withRpsYD25were identified and validated to be diagnostic markers. Combined with the resistance reaction to multipleP. sojaeisolates, seven of 178 soybean genotypes were detected to containRpsYD25using the five co-segregated SSR markers. The soybean genotypes carryingRpsYD25and the developed co-segregated markers can be effectively applied in the breeding forP. sojaeresistance in China.

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