Phospholipase C gamma (PLC gamma) regulates soluble trehalase in the 20E-induced fecundity ofApolygus lucorum

文献类型： 外文期刊

第一作者： Tan, Yong-An

作者： Tan, Yong-An;Zhao, Jing;Xiao, Liu-Bin;Jiang, Yi-Pin;Zhao, Xu-Dong;Hao, De-Jun;Sun, Hou-Jun

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关键词： Apolygus lucorum; phospholipase C; reproduction; soluble trehalase; 20-hydroxyecdysone; vitellogenin

期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）

ISSN： 1672-9609

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收录情况： SCI

摘要： Apolygus lucorumis the dominant pathogenic insect attackingBacillus thuringiensis(Bt) cotton in China. Additionally, 20-hydroxyecdysone (20E) has important functions in many biological processes, including insect reproduction. Phospholipase C (PLC), which is an essential enzyme for phosphoinositide metabolism, is involved in 20E signal transduction, but its function in 20E-mediated reproduction inA. lucorumremains unclear. In this study, 20E increasedAlPLC gamma transcription as well as the abundance and activity of the encoded protein during molting and metamorphosis. The 20E treatment also induced the considerable accumulation of two second messengers, inositol triphosphate and diacylglycerol. The expression levels of genes encoding vitellogenin (AlVg) and soluble trehalase (AlTre-1) were similar to those ofAlPLC gamma, and were upregulated in response to 20E. The silencing ofAlPLC gamma resulted in downregulated expression ofAlTre-1andAlVg. However, the silencing ofAlTre-1andAlVgdid not affectAlPLC gamma expression. Moreover, the silencing ofAlVgdid not alterAlTre-1expression. Furthermore, an examination of the insect specimens indicated thatAlPLC gamma is required for female adult reproduction, and that downregulated expression of this gene is associated with decreases in fecundity, adult longevity, and egg hatching rate as well as delayed oocyte maturation. We propose that 20E regulatesAlTre-1expression viaAlPLC gamma and affectsVgexpression as well as ovary development to facilitate the reproductive activities ofA. lucorumfemales.

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