The Cds.71 onTMS5May Act as a Mutation Hotspot to Originate a TGMS Trait in Indica Rice Cultivars

文献类型： 外文期刊

第一作者： Tan, Yanning

作者： Tan, Yanning;Sun, Xuewu;Sun, Zhizhong;Yu, Dong;Liu, Hai;Yuan, Dingyang;Tan, Yanning;Sun, Xuewu;Sun, Zhizhong;Yu, Dong;Liu, Hai;Yuan, Dingyang;Fang, Baohua;Sheng, Xiabing;Li, Zheli;Duan, Meijuan;Liu, Ling

作者机构：

关键词： rice (Oryza sativaL; ); two-line hybrid rice; thermo-sensitive genic male sterility gene 5 (tms5); thermo-sensitive genic male sterility (TGMS); mutation hotspot; T98S; unbalanced allele frequency; indica

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： The genetms5, which controls thermo-sensitive genic male sterility (TGMS), has been widely used in two-line hybrid rice breeding in China. Thetms5lines have two sources, namely, AnnongS-1 (AnS) and Zhu1S (ZhS) and, interestingly, are commonly subject to an alteration at cds.71. However, whether cds.71 acts as a mutation hotspot is unknown. Herein, anothertms5mutant named T98S (induced from T98B by irradiation) was used to explore this. First, the gene oftms(t)responsible for T98S was fine-mapped on chromosome 2 based on an F(2)group of T98S/R893. In T98S, the candidate geneTMS5(LOC_Os02g12290.1) mutated at cds.71 with a transversion from cytosine (C) to adenine (A), as also observed in AnS and ZhS. Moreover, the entire coding sequence ofTMS5from T98B converted T98S from sterile to fertile byAgrobacterium tumefaciens-mediated transformation, confirming that T98S is controlled bytms5. Next, detection on nearly 40,000 single nucleotide polymorphisms (SNPs) on Rice 56K SNP Array revealed T98S was 99.99% similar to T98B but only 72.84% and 77.47% similar to AnS and ZhS, respectively, demonstrating that T98S originated from T98B rather than from existingtms5lines. Furthermore, the cds.70 was found to exist as a T/G haplotype, and it was T rather than G that helped to induce a TGMS trait. The T frequency was 67.52% in indica rice but decreased to 1.75% in japonica rice in 2,644 cultivars tested, which partly explains whytms5mutants were mostly found in indica lines. Our findings provide evidence that cds.71 may act as a mutation hotspot and clues for breeding TGMS lines in a more efficient way.

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