Bioinformatics analysis of aberrantly expressed exosomal lncRNAs in oral squamous cell carcinoma (CAL-27 vs. oral epithelial) cells

文献类型： 外文期刊

第一作者： Jin, Jiajia

作者： Jin, Jiajia;Huang, Zixiao;Lu, Xiaoyan;Wu, Shengrong;Jia, Mei'e;Li, Xin;He, Xiangyi;Jin, Jiajia;Li, Zhiyong;He, Xiangyi

作者机构：

关键词： oral squamous cell carcinoma; exosome; long non-coding RNAs; bioinformatics

期刊名称：ONCOLOGY LETTERS （ 影响因子：2.967； 五年影响因子：2.575 ）

ISSN： 1792-1074

年卷期： 2020 年 20 卷 3 期

页码：

收录情况： SCI

摘要： Oral squamous cell carcinoma (OSCC) is the most prevalent form of malignant tumour in the oral cavity and its early detection is critical for improving the prognosis of affected patients. The present study aimed to isolate and confirm exosomes derived from the supernatant of the OSCC cell line CAL-27 and human oral epithelial cells (HOECs), analyze long non-coding RNA (lncRNA) expression profiles and determine the diagnostic value based on bioinformatics analyses. The results indicated that the particles isolated from the supernatant of CAL-27 and HOECs were either round or oval, had a size range of 30-150 nm and were enriched with ALG-2 interacting protein X (ALIX) and tumour susceptibility 101 proteins (TSG101). These characteristics confirmed that these particles were exosomes. Three lncRNAs (NR-026892.1, NR-126435.1 and NR-036586.1) were selected as potential diagnostic biomarkers for OSCC. The expression levels of the selected lncRNAs were significantly different in CAL-27-exo vs. HOEC-exo, as well as in whole cells (CAL-27 vs. HOECs) (P<0.001). The expression levels of the three lncRNAs confirmed by quantitative PCR were consistent with the sequencing data. In conclusion, various lncRNAs were aberrantly expressed between cancerous and non-cancerous exosomes, suggesting that they may serve as biomarkers for cancer.

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