An ERF transcription factor enhances plant resistance toMyzus persicaeandSpodoptera litura

文献类型： 外文期刊

第一作者： Wu, Juan

作者： Wu, Juan;Gao, Hao;Zhu, Xiwu;Wu, Juan;Li, Defang

作者机构：

关键词： Myzus persicae; Spodoptera litura; ERF transcription factor; BrERF11b; plant resistance

期刊名称：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT （ 影响因子：1.632； 五年影响因子：2.029 ）

ISSN： 1310-2818

年卷期： 2020 年 34 卷 1 期

页码：

收录情况： SCI

摘要： Aphids are sap-sucking insects and are pests that cause substantial damage to many crops worldwide.Spodoptera liturais a leaf-chewing insect and poses a severe threat to many species of vegetable and cotton plants. In this paper, we studied an ethylene-responsive factor (ERF) that enhances plant resistance to sap-sucking and leaf-chewing insects.ERF11-like was obtained from Chinese cabbageviareverse transcription-polymerase chain reaction and was termedBrERF11b. The putative protein encoded by theBrERF11bgene includes 168 amino acid residues and contains an EAR motif. The plasmid pBI121-BrERF11bwas constructed and transformed into tobacco plants (Nicotiana tabacumcv. K326 andN. benthamiana) by theAgrobacterium-mediated transformation method. The function of theBrERF11bgene in the resistance of the transgenic plants toMyzus persicaeandS. liturawas assayed. The results showed that the weights of theS. lituralarvae fed the transgenic plant line were significantly reduced from 28 to 76% compared to those of theS. lituralarvae fed the control plants (wild type), and the transgenic plant line KLine12 significantly reduced the survival rate ofS. lituraby 62.5%. The colony growth of the apteral aphids fed the transgenic plant line was significantly reduced from 22.5 to 63.4% compared to that of the apteral aphids fed the control plants. Compared to control plants, the transgenic plant line significantly reduced the survival rate of the aphids from 46 to 55%. These results first indicate that theBrERF11bgene significantly enhances plant resistance toS. lituraandM. persicae.

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