Characterization of a New Bifunctional and Cold-Adapted Polysaccharide Lyase (PL) Family 7 Alginate Lyase fromFlavobacteriumsp.
文献类型： 外文期刊
第一作者： Zhou, Hai-Xiang
作者： Zhou, Hai-Xiang;Wang, Feng-Long;Xu, Shan-Shan;Yin, Xue-Jing;Li, Yang
作者机构：
关键词： alginate lyase; bifunctional; cold-adapted; Flavobacteriumsp; S02; Yarrowia lipolytica
期刊名称：MARINE DRUGS （ 影响因子：5.118； 五年影响因子：5.951 ）
ISSN：
年卷期： 2020 年 18 卷 8 期
页码：
收录情况： SCI
摘要： Alginate oligosaccharides produced by enzymatic degradation show versatile physiological functions and biological activities. In this study, a new alginate lyase encoding genealyS02fromFlavobacteriumsp. S02 was recombinantly expressed at a high level inYarrowia lipolytica, with the highest extracellular activity in the supernatant reaching 36.8 +/- 2.1 U/mL. AlyS02 was classified in the polysaccharide lyase (PL) family 7. The optimal reaction temperature and pH of this enzyme were 30 degrees C and 7.6, respectively, indicating that AlyS02 is a cold-adapted enzyme. Interestingly, AlyS02 contained more than 90% enzyme activity at 25 degrees C, higher than other cold-adapted enzymes. Moreover, AlyS02 is a bifunctional alginate lyase that degrades both polyG and polyM, producing di- and trisaccharides from alginate. These findings suggest that AlyS02 would be a potent tool for the industrial applications.
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