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Characterization of a New Bifunctional and Cold-Adapted Polysaccharide Lyase (PL) Family 7 Alginate Lyase fromFlavobacteriumsp.

文献类型：外文期刊

第一作者： Zhou, Hai-Xiang

作者： Zhou, Hai-Xiang;Wang, Feng-Long;Xu, Shan-Shan;Yin, Xue-Jing;Li, Yang

作者机构：

关键词： alginate lyase; bifunctional; cold-adapted; Flavobacteriumsp; S02; Yarrowia lipolytica

期刊名称：MARINE DRUGS （影响因子：5.118；五年影响因子：5.951 ）

ISSN：

年卷期： 2020 年 18 卷 8 期

页码：

收录情况： SCI

摘要： Alginate oligosaccharides produced by enzymatic degradation show versatile physiological functions and biological activities. In this study, a new alginate lyase encoding genealyS02fromFlavobacteriumsp. S02 was recombinantly expressed at a high level inYarrowia lipolytica, with the highest extracellular activity in the supernatant reaching 36.8 +/- 2.1 U/mL. AlyS02 was classified in the polysaccharide lyase (PL) family 7. The optimal reaction temperature and pH of this enzyme were 30 degrees C and 7.6, respectively, indicating that AlyS02 is a cold-adapted enzyme. Interestingly, AlyS02 contained more than 90% enzyme activity at 25 degrees C, higher than other cold-adapted enzymes. Moreover, AlyS02 is a bifunctional alginate lyase that degrades both polyG and polyM, producing di- and trisaccharides from alginate. These findings suggest that AlyS02 would be a potent tool for the industrial applications.

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